高血压病肝火亢盛证与阴虚阳亢证的血清代谢组学比较研究

Comparative Study of Serum Metabonomics of Hypertension of Liver-Fire Hyperactivity Syndrome and Yin-Deficiency and Yang-Hyperactivity Syndrome

目的分析比较高血压病肝火亢盛证、阴虚阳亢证的不同生物标志物群,探索高血压病中医"证"的分子物质基础。方法运用气相色谱飞行时间质谱(GC/TOF-MS)技术进行血清代谢组学分析。结果①高血压病阴虚阳亢证组天门冬氨酸氨基转移酶、甘油三酯显著高于肝火亢盛证组(P<0.05)。②高血压病肝火亢盛证组与阴虚阳亢证组在主成分得分三维图上有一定的差异,能鉴定出的差异化合物有:柠檬酸盐、草酸盐、二氧化庚酸、甘油酸盐、软酸盐、乙醇胺、肌醇、油酸、硬脂酸、门冬氨酸、苏丁糖酸、软脂酸。结论代谢组学方法能够区分高血压病肝火亢盛证组与高血压病阴虚阳亢证组,两证型间确实存在生物标志物的差异,有望为高血压病的中医辨证分型提供一定的客观依据。

OBJECTIVE To analyze and compare the different biomarker groups of liver-fire hyperactivity syndrome and yin -deficiency and yang-hyperactivity syndrome of hypertension and explore molecular basis of TCM syndrome of hypertension.METHODS The gas chromatography-time of flight mass spectrography （GC-TOFMS） technology was employed to analyze the serum metabonomics.RESULTS ①Glutamic oxalacetic transaminase （GOT） and triacylglycerol in group of yin-deficiency and yang-hyperactivity syndrome of hypertension is markedly higher than the group of liver-fire hyperactivity syndrome （P＜0.05）.②Groups of liver-fire hyperactivity syndrome and yin-deficiency and yang-hyperactivity syndrome in hypertension are different in the principal component scores three-dimensional diagram.The different compounds which can be identified include citrate,oxalate,dioxide heptanoic acid,glycerate,soft hydrochloric acid,ethanol amine,inositol,oleic acid,stearic acid,aspartate,threose acid,palmitic acid.CONCLUTION Metabonomics method can distinguish group of liver-fire hyperactivity syndrome of hypertension and group of yin-deficiency and yang-hyperactivity syndrome of hypertension.The difference of biomarker does exist in the two syndromes and is expected to provide a certain objective basis for TCM syndrome differentiation typing of hypertension.