六味地黄汤对慢性肾衰大鼠肾组织中HIF-1α、CTGF表达的影响

Effects of Liuwei Dihuang Decoction on Expression of HIF-1α and CTGF in Kidney Tissues of Chronic Renal Failure Rats

目的探讨HIF-1α、CTGF在慢性肾衰大鼠肾组织中的表达及作用,观察六味地黄汤对其表达的影响。方法用5/6肾切除法制作慢性肾衰模型,分假手术组、模型组和六味地黄汤组。造模后12周,检测各组肾功能指标;HE染色观察肾组织病理变化,Masson染色观察肾组织胶原的沉积;免疫组化法和RT-PCR法分别检测HIF-1α、CTGF在肾组织中的蛋白水平和基因表达。结果与假手术组比较,模型组肾组织中HIF-1α、CTGF蛋白水平和基因表达均明显升高(P<0.01)。与模型组比较,六味地黄汤组肾组织中HIF-1α、CTGF蛋白水平和基因表达均明显降低(P<0.01)。结论 HIF-1α上调CTGF的表达促进肾间质纤维化,六味地黄汤可下调HIF-1α的表达,从而抑制肾间质纤维化。

OBJECTIVE To determine the expressions and effects of HIF-1α and CTGF in kidney tissues of chronic renal failure rats,and to observe the influence of Liuwei Dihuang Decoction on the expression.METHODS The rat models of chronic renal failure were induced by excising 5/6 kidney,which were divided into sham operation group,model group and Liuwei Dihuang Decoction group.After 12 weeks,each index of renal functions was detected.HE was stained to observe renal pathological changes and Masson to observe the deposition of renal tissue collagen; immunohistochemical method and RT-PCR were applied to detect protein levels and gene expression of HIF-1α and CTGF in renal tissues respectively.RESULTS Compared with sham operation group,the protein level and gene expression of HIF-1α and CTGF in renal tissues in model group were significantly elevated（P ＜0.01）.Compared with model group,the protein level and gene expression of HIF-1α and CTGF in renal tissues in treatment group were significantly reduced（P＜0.01）.CONCLUSION HIF-1α can promote renal interstitial fibrosis by up-regulating CTGF expression while Liuwei Dihuang decoction may attenuate renal interstitial fibrosis by reducing HIF-1α expression.