血脉通颗粒对动脉粥样硬化小鼠基质金属蛋白酶及其抑制物的影响

Effects of Xuemaitong Granule on Expression of Matrix Metalloproteinase(MMP) and Tissue Inhibitors(TIMP) of Carotid Atherosclerosis

目的探讨血脉通颗粒对动脉粥样硬化(atherosclerosis,AS)小鼠基质金属蛋白酶(MMPs)的生成和(或)促进基质金属蛋白酶抑制剂(TIMPs)表达的影响。方法 33只8周龄apoE基因敲除小鼠饲喂以高脂饲料13周确定AS斑块形成后随机分为血脉通颗粒干预组、西药立普妥干预组和AS模型组,血脉通组和立普妥组给予灌胃给药,每日1次,对照组给予等量生理盐水,6周后处死。应用ELISA、Q-PCR和Western blotting检测3组小鼠MMP2、MMP9、TIMP-1和TIMP-2的表达水平。结果血脉通组和立普妥组小鼠血清MMP2和MMP9表达水平明显低于模型组(P<0.05),两个干预组之间没有显著区别(P>0.05);立普妥组TIMP-2含量明显高于血脉通颗粒组和模型组(P<0.05),血脉通颗粒组和模型组之间没有显著区别(P>0.05);相反,血脉通颗粒组TIMP-1含量明显高于立普妥组(P<0.05),立普妥组和模型组之间没有显著区别(P>0.05),主动脉斑块进行Q-PCR和Western blotting检测得到类似结果。结论血脉通颗粒能降低AS小鼠MMP2和MMP9的表达水平并上调TIMP-1的表达水平。

OBJECTIVE To investigate the effects of Xuemaitong Granules on expression of matrix metalloproteinases （MMPs） and matrix metalloproteinase inhibitors （TIMPs） in apoE-deficient mice.METHODS Male ApoE-deficient mice （n=33,aged 8 weeks,18 to 20 g） on a C57BL/6J background,fed a high fat diet for 13 weeks.After confirming the atherosclerosis plaque formed,mice randomly divided into three groups：Xuemaitong Granules group,lipitor group and control group.Af ter six weeks,venous blood and aorta were taken.The expression of MMP-2,MMP-9,TIMP-1 and TIMP-2 were measured by ELISA,Q-PCR and Werstern blot,respectively.RESULTS Compared with control group,the serum concentration of MMP2 and MMP9 significantly decreased （P＜0.05） in two intervention groups and there was no significant difference（P＞0.05） in them.The serum concentration of TIMP1 in Xuemaitong granules intervention group is much higher than in the other groups.However,The serum concentration of TIMP2 in the lipitor intervention group is much higher than that in the other groups.The same results were also detected by Q-PCR and Western blot in the aortic plaques.CONCLUSION Xuemaitong Granules can reduce the expression of MMP2 and MMP9 and up-regulate the expression of TIMP-1 in apoE-deficient mice.