摘要
为了优化生物破乳剂生产的发酵过程,提高生物破乳剂的产量,对破乳剂产生菌Bacillus mojavensis XH-1的分批发酵过程进行了研究.根据破乳剂产生菌XH-1发酵过程的特点,在Logistic方程和Luedeking-Piret方程的基础上,建立了菌株XH-1发酵过程中菌体生长、基质消耗和蛋白类破乳剂形成的动力学方程.采用Origin 7.5软件对试验数据进行处理,得到了破乳菌XH-1的分批发酵动力学模型参数.结果表明:模型预测值和试验值吻合较好,所建立的方程能较好地预测其实际发酵过程,具有很好的适用性,对生物破乳剂的发酵生产具有一定的指导意义.
To optimize the fermentation process of demulsifying bacteria Bacillus mojavensis XH - 1, the growth characteristics were investigated to analyze the fermentation kinetics. Based on the characteristics of bacteria XH - 1, Logistic equation and Luedeking-Piret equation, the kinetic models were established to describe the fermentation features of strain growth, substrate consumption and protein demulsifier generation. The experiment data was processed with Origin 7.5 software to obtain batch fermentation kine- tics model parameter. The good agreement of prediction values with the experimental results shows that the proposed kinetic models are suitable for predicting the actual fermentation process.
出处
《江苏大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2013年第4期455-459,共5页
Journal of Jiangsu University：Natural Science Edition
基金
国家自然科学基金资助项目(41271504)
中国博士后基金资助项目(20100480174)
黑龙江省青年基金资助项目(QC2010092)
关键词
破乳剂产生菌
分批发酵
蛋白类破乳剂
发酵动力学
动力学模型
bio-demulsifier producing bacteria
fed-batch fermentation
protein demulsifier
fermentation kinetics
kinetic model
