硒增强T淋巴细胞抗大肠癌作用的实验研究

Study of Enhancing Effect of Selenium on Anti-turmor Function of T-lymphocyte in Colonic Cancer Cell

目的：探讨硒对T淋巴细胞抗结肠癌细胞作用的影响。方法：以半胱氨酸硒作为影响因素，人T淋巴细胞为效应细胞，人结肠癌细胞（LoVo细胞株）为靶细胞，采用MTT比色法和凋亡细胞荧光计数检测凋亡细胞的数量；同时检测上清液中肿瘤坏死因子（TNF）α、超氧化物歧化酶（SOD）和丙二醛（MDA）含量的变化。结果：半胱氨酸硒在一定浓度范围内（0.5μg／ml～1．0μg／ml）可增强人T淋巴细胞的抗肿瘤活性（p＜0.05），促进人结肠癌细胞凋亡，且肿瘤细胞凋亡比例上升（P＜0．05）；T淋巴细胞抗肿瘤作用的增强与上清液中TNF-α水平增高有关（P＜0.05），与SOD和MDA无关（P＞0.05）。结论：硒可提高T淋巴细胞的抗肿瘤作用。

Background/Aims: To study whether selenocystine (Se) could enhance the anti-turmor function of T-lymphocyte in colonic cancer cell. Methods: Selenocystine is a factor influencing human T-lymphocyte which acts as an effector cell and human colonic tumor cell (LoVo cell) as a target cell. This study was allocated into four groups: control, Se 0.5μg/ml, Se 1.0μg/ml and Se 2.μg/ml. The ratio of effector cell to target cell was 20：1. MTT and acridine orange were used for quantitative measurement,TNF-α, SOD and MDA were also measured. Results: The selenium enhanced the anti-tumor effect of T-lymphocyte and induced LoVo cells apoptosis within a certain concentration range of 0.5μg/ml^1.0μg/ml, and this effect was time- and dose-dependent (P<0.05). Enhancement of T-lymphocyte antitumor effect was related to TNF-7 (P<0.05), but not to SOD and MDA (P>0.05). Conclusions:Selenocystine can enhance the anti-turmor function of T-lymphocyte in colonic cancer cell, which is related to TNF-α, but not to SOD and MDA.