小鼠离体小肠平滑肌实验方法的研究

Methodology Study on In Vitro Smooth Muscle of Mice Small Intestinal

目的:考察影响小鼠离体小肠平滑肌实验结果的主要因素及评价指标,优化实验方案。方法:以小鼠离体胃肠道平滑肌为实验材料,采用单因素实验考察影响实验结果的各主要因素,如营养液、取样长度、取样部位、离体时间等。结果:台氏液中的离体小肠最稳定,存活时间最长;取样长度为1cm时小肠收缩较稳定;取样部位为距离幽门1-5cm段的小肠节律性好,收缩稳定;小肠活性维持稳定时间约为60min。结论:采用台氏液作为营养液,取距幽门1-5cm的小肠1cm进行离体平滑肌实验,60min内能得到较稳定的实验数据。

Objective： To study and evaluate the experimental factors that may affect the results on mice small intestinal smooth muscle in vitro. This research can help in selecting better experiment protocol on this area. Methods： One-way ANOVA is adopted to evaluate the influential factors on the smooth muscle in vitro, such as nutrient solution, sampling length and sampling location, in vitro time and so on. Results： The small intestinal muscle is the stablest, and the life time is the longest in Tyrode＇ s solution; the contraction of small intestine is stable in lcm length; the sample sected 1-5cm apart from pylorus has better rhythmicity and contraction stability; the sample can keep stable activity in 60 min. Conclusion： The optimum results are follows： Tyrode＇ s solution as nutrient, lcm of small intestine sected from 1-5cm distance ofpylorus, and datawith 60min.