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CHO DG44细胞无血清培养基关键组分的优化与替代 被引量：2

Optimization and Substitution of Key Components for Suspension Culture of Chinese Hamster Ovary (CHO DG44) Cells in Serum Free Medium
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摘要 以悬浮CHO DG44细胞为对象,采用central composite响应面分析法,以活细胞密度为评价指标,确定了3种对细胞生长有明显促进作用的培养基关键组分——胰岛素、转铁蛋白及亚硒酸钠(简称ITS)的最优浓度及其相互影响模式,并设计了一种有效的低成本ITS替代混合物。同时在添加25 mg/L硫酸葡聚糖条件下成功解决细胞高密度悬浮培养的成团问题,为CHO细胞低成本无血清培养基的研制提供了理论依据。 With suspension Chinese Hamster Ovary cell lines（CHO DG44） as the object of study,a serum free medium for the cultivation of CHO cells in suspension was formulated by using central composite response surface methodology.As Insulin,transferrin,and sodium selenite（ITS） were identified as the most significant factors,the response surface methodology was used to determine the optimal levels of these factors,where the evaluating index was viable cell density.And an inexpensive substitution of ITS for CHO cells suspension culture was formulated.Also the clumping of cells was resolved by adding 25 mg/L dextran sulfate to medium,which provided a basis for inexpensive serum free medium for CHO cells.
作者 俞锦锋 陈慧萍 缑向楠 芦海姗
机构地区 南昌大学转化医学研究院 南昌大学环境与化学工程学院
出处 《南昌大学学报（工科版）》 CAS 2013年第2期125-129,175,共6页 Journal of Nanchang University(Engineering & Technology)
基金 国家"十二五"专项课题资助项目(2011ZX09401-023) 江西省研究生创新资金资助项目(YC2011-S014)
关键词 CHO DG44细胞 响应面分析 硫酸葡聚糖 无血清培养基 CHO DG44 cells response surface methodology dextran sulfate serum free medium
分类号 Q813.1 [生物学—生物工程]
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参考文献13

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共引文献8

同被引文献64

  • 1赵亮,朱明龙,张旭,谭文松.钙离子对293细胞结团和生长的影响[J].生物工程学报,2005,21(3):482-485. 被引量：4
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南昌大学学报（工科版）
2013年 第2期

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