肺癌组织MTS1/p16基因缺失的研究

Deletion of MTS1/p16 gene in human lung cancers

目的:研究MTS1/p16基因在人肺癌组织中的变化。方法:应用Southern杂交分析了46例肺癌组织标本,PCR技术分析89例肺癌组织标本。结果:Southern杂交检测p16基因的缺失率为17.4％(8/46),PCR检测p16基因缺失率为25.8％(23/89)。

Objective: To investigate the alteration of MTS1/pl6 gene in human lung cancer tissue. Methods: Southern blotting study on 46 cases and PCR on 89 cases of lung cancers were performed. Results: High rate of gene deletion was detected by southern blotting in 8 of 46 fresh tumor tissues (17.4%) and by PCR in 23 of 89 lung cancers 25. 8% . Conclusions: MTS1/pl6 gene alteration may play the important role in the development of lung cancer.