中国南瓜海南农家品种间的遗传特异性分析和DNA指纹图谱构建

Analysis of Genetic Specificity and DNA Fingerprinting Establishment for the Hainan Island Landraces of Cucurbita moschata

在收集中国南瓜海南农家品种的基础上,应用ISSR和SRAP标记技术对28份海南农家品种间的遗传特异性进行了分析,并构建指纹图谱,为中国南瓜海南农家品种鉴定、评价、保护和利用提供科学依据。结果表明,所供试的品种间存在显著的遗传特异性,具有特殊的遗传基础或背景,所筛选的6个ISSR引物和11对SRAP引物共产生了10个特异标记和11条唯一缺失带;应用ISSR引物组合UBC807/UBC814/UBC844/UBC868和UBC808/UBC814/UBC844/UBC868,以及SRAP引物组合Me1/Em2+Me1/Em10+Me2/Em3和Me1/Em1+Me1/Em10+Me8/Em3分别绘制了4个28份中国南瓜海南农家品种的DNA指纹图谱,所构建的DNA指纹图谱直观、简单。ISSR标记和SRAP标记技术可有效应用于中国南瓜海南农家品种DNA指纹图谱的构建和遗传特异性鉴定。

Based on the collection of Hainan island landraces of Cucurbita moschata,the present study was aimed to analyze their genetic specificity and establish DNA fingerprinting for 28 landraces by inter-simple sequence repeat（ISSR） and sequence-related amplified polymorphism（SRAP）,which would provide applicable guidance for identification,evaluation,protection,and utilization of Hainan island landraces of C.moschata.The results showed that genetic specificity was significant among the 28 landraces with special genetic background,10 specific markers and 11 unique-lack bands scored were produced by 6 ISSR primers and 11 SRAP primer pairs.And four DNA fingerprintings for the 28 landraces were developed by two ISSR primer combinations and two SRAP primer combinations,i.e.UBC807/UBC814/UBC844/UBC868,UBC808/UBC814/UBC844/UBC868,Me1/Em2 ＋ Me1/Em10 ＋ Me2/Em3,Me1/Em1 ＋ Me1/Em10 ＋ Me8/Em3,respectively.All the DNA fingerprintings developed were intuitionistic and simple.ISSR and SRAP molecular markers could be effectively used in the analysis of genetic specificity and DNA fingerprinting establishment for the landraces of C.moschata.