中线恶性淋巴瘤临床病理和免疫组化特征研究

A Study on the Pathological and Immunohistochemical Characteristics of Malignant Lymphoma in Midline

目的 :探讨头面中线非何杰金淋巴瘤 (中线NHL)的临床病理特点及免疫表型。方法 :采用免疫组化LSAB法 ,选用单克隆抗体CK、LCA、UCHL1、L2 6检测 2 8例中线NHL ,4例鼻咽癌(NPC)和 2例坏死性鼻腔炎的组织表型抗原。结果 :2 8例中线NHL中 ,2 7例阳性表达LCA(白细胞共同抗原 ) ;2 0例 (71.4 % )UCHL1阳性 ,为T细胞淋巴瘤 ;8例 (2 8.6 % )L2 6阳性 ,为B细胞淋巴瘤。无 1例表达CK(Cytokeratin ,细胞角蛋白抗原 )。 4例NPC中的肿瘤细胞均表现较强的CK阳性而LCA呈阴性。 2例坏死性鼻腔炎及NPC的病灶中可见一些散在的细胞呈现LCA、UCHL1、L2 6阳性。结论 :中线NHL以T细胞淋巴瘤多见。

Objective:To study the clinical pathological characteristic and immunophenotype of Midline Non Hodgkin's lymphoma(NHL).Method: A immunohistochemical method LSAB,was applied and monoclonal antibodies CK, LCA,UCHL1 and L26 were used to detect the corresponding antigens in 28 cases with Midline NHL,4 cases with NPC and 2 cases of necrotic nasal cavity inflammation. Result:LCA was expressed in 27 of 28 cases with Midline NHL. 20 of them were proved to be T cell Lymphoma according to their positive expression of UCHL1 ,the rest (8 cases) were proved to be B cell Lymphoma ,in that L26 was positively expressed .In all four cases with NPC , CK was expressed in the tumor cells but LCA was negative .It was also found that LCA,UCHL1 and L26 were expressed in dispersed cells in the focus from patients with NPC and necrotic nasal cavity inflammation .Conclusion: T cell lymphoma is the main pathological type in midline NHL.