利用基因芯片技术筛选早期乳腺癌的差异表达基因

Differential gene expression pattern in early breast cancer by microarray analytical procedures

目的 比较相同病理类型和临床分期预后不同的早期（Ⅰ、Ⅱ期）乳腺癌样本的基因表达差异,寻找有显著差异的基因,探索与早期乳腺癌预后有关的基因分型.方法 用Agilent4×44K人全基因组Oligo芯片对47例早期乳腺癌患者的组织样本,结合其预后好、差数据,根据其临床预后的不同分为对照组（预后好）24例和实验组（预后差）23例,进行差异基因表达分析;采用Real-time PCR技术,对两组乳腺癌样本中差异表达的基因进行验证.结果 基因芯片检测分析发现,实验组样本比对照组样本有差异表达基因126个,其中60个基因显著上调,66个基因显著下调（差异均在2倍以上）.结论 不同预后的早期乳腺癌样本中,基因表达存在显著差异,早期乳腺癌的预后与这些基因的表达有关.

Objective Analysis of the same pathological type,clinical stage （Ⅰ-Ⅱ） but the expression of breast cancer prognosis of different genetic differences,screened the gene combination meaningful,look for related with the prognosis of breast cancer gene.Methods The Agilent 4 × 44 K human whole genome Oligo chip were used to screen 47 patients with early breast cancer tissue samples,combined with their difference of prognosis,divided into control group （poor prognosis） and experimental group （good prognosis） for differential gene expression analysis.Results Microarray analysis revealed that the experimental group sample than in the control group samples are differentially expressed genes 126,of which 60 genes significantly up-regulated and 66 genes were significantly down-regulated （differences were more than 2 times）.Conclusions Different samples of early breast cancer prognosis,there are significant differences of gene expression,the prognosis of early breast cancer is associated with the expression of these genes.