用于质谱分析的丹参蛋白/多肽序列数据库的构建

Contrucion of Salvia miltorrhiza Protein/Peptide Database for Mass Spectrometry Analysis

丹参是我国传统中药材,目前已从中鉴定出数十种具有药用功效的化合物成分。针对丹参药用成分合成机制的蛋白组学研究可为丹参品种遗传改良提供理论依据。本研究以Illumina高通量测序手段获取的不同组织丹参转录组拼接序列为基础,构建了可用于蛋白组学分析的丹参特异的蛋白/多肽序列数据库,为后期开展丹参蛋白组学研究提供了基础。

Salvia miltorrhiza Bge is a medicinal herb in traditional Chinese medicines.Until recently,dozens of compound ingredients with medicinal properties have been identified from S.miltorrhiza.Proteome analysis on medicinal ingredients synthesis mechanisms could provide a theoretical basis for S.miltorrhiza genetic improvement.In this study,a S.miltorrhiza specific protein/peptide sequence database was constructed using Illumina high-throughput transcriptome sequencing data of multiple types of S.miltorrhiza tissues.The database could act as a key component to carry out the proteome analysis in S.miltorrhiza.