灵芝菌对茯苓药材的生物转化研究

Studies on the Biotransformation of Poria cocos by Ganoderma lucidum

目的:利用灵芝菌对中药材茯苓药材进行固态生物转化,研究转化产物中总多糖和总三萜量的变化情况。方法:采用微波法提取茯苓及转化产物中的总多糖并采用苯酚-硫酸法进行含量测定;采用超声法提取茯苓及转化产物中总三萜并用香草醛-高氯酸法进行含量测定。结果:经生物转化后产物中的总多糖含量明显高于未经转化的茯苓药材(P<0.01),而总三萜含量明显升高(P<0.01),说明灵芝菌的生物转化过程改变了茯苓药材的原始特性;茯苓对灵芝生长具有促进作用,表现为灵芝菌丝生长旺盛未受抑制、转化产物中总多糖及总三萜含量显著高于茯苓原药材。结论:利用灵芝菌对中药茯苓进行生物转化来提高总多糖和总三萜含量,具有方法简单、成本低等优点,在中药现代化中有较高的应用价值。

Objective： To study the biotransformation of Poria cocos by Ganoderma lucidum and the changes of total polysaccharide and total triterpenes contents of the biotransformation products.Method： The experiment uses the microwave method to extract and sulfuric acid-phenol method to measure total polysaccharide content.And the ultrasonic wave method was used to extract total triterpenes and the content was measured by vanillin perchloric method.Result： After treatment by G.lucidum,the total polysaccharide and triterpenes content of the biotransformation products were significantly higher than the non-conversed P.cocos（P ＆lt;0.01）,showing that the original performance of P.cocos was changed by way of biotransformation of G.lucidum;P.cocos had a promotion effect on the growth of G.lucidum.Conclusion： Biotransformation of P.cocos for Increasing the total polysaccharide and triterpenes contents by using G.lucidum has advantages of simple,low cost and so on.This method has a certain application value in modernization of Chinese traditional medicine.