金银花中表达基因密码子偏好性分析

Analysis on Codon Usage Bias of the Expressed Genes in Lonicera japonica Thunb.

密码子是生物体中重要的遗传信息载体,经过长期进化,不同的生物体对同一密码子形成了一定程度的使用偏好性。本研究对已获得的金银花转录组进行基因预测,利用CodonW程序对所有含有完整阅读框的预测基因,以及参与绿原酸合成的羟基化肉桂酰转移酶(HQT)基因进行密码子偏好性分析。并将HQT基因与大肠杆菌、酵母和拟南芥全基因组的密码子使用频率进行比较,为在细菌、真菌和植物中表达金银花中HQT基因,或通过合成生物学手段异源合成绿原酸奠定了前期研究基础。

Codon is an important carrier of genetic information in organisms.In evolution,codon bias is formed in many organisms.In this study,the analysis of expressed genes in Lonicera japonica Thunb.is presented.The codon bias of genes with complete coding region is analyzed through CodonW.And we calculated the codon usage bias of hydroxycinnamoyl-CoA： quinate hydroxycinnamoyl transferase（HQT） genes.The results showed that L.japonica were bias toward the synonymous codons with A and T at the third codon position.