摘要
目的:研究维药神香草对COPD小鼠Th1/Th2和Th17/Treg平衡的影响,探讨神香草治疗COPD的免疫作用机制。方法:复制前期熏烟法建立COPD小鼠模型,将小鼠随机分为正常组、生理盐水组、神香草高、中、低剂量组。采用酶联免疫吸附法检测小鼠血清中IFN-γ、IL-4、IL-17及IL-10的水平。结果:与正常组比较,COPD模型组小鼠血清IL-4、IL-17水平明显升高(P<0.05),IFN-γ、IL-10的水平明显降低(P<0.05);神香草治疗后,与模型组比较,小鼠血清IL-4、IL-17水平明显降低,IFN-γ和IL-10水平明显升高。相关性分析表明IFN-γ与IL-4,IL-17与IL-10都呈负相关。结论:神香草在纠正Th1/Th2和Th17/Treg失衡方面发挥了一定的作用。
This article was aimed to study the Uygur medicine Hyssopus officinalis L.on the balance of Th1/Th2 and Th17/Treg of chronic obstructive pulmonary disease（COPD） mice in order to explore the mechanism of its treatment of COPD.The pre-fumigation method was copied to establish a mouse model of COPD.Mice were randomly divided into the normal control group,saline group and water extract of H.officinalis L.high,middle and low dose group.The level of serum IFN-γ,IL-4,IL-17 and IL-10 were detected with ELISA.The results showed that compared with the normal control group,the serum IL-4 and IL-17 level of the COPD model group were significantly higher（P ＆lt; 0.05）,and the serum IFN-γ and IL-10 level were significantly lower（P ＆lt; 0.05）.After drug intervention,the IL-4 and IL-17 level were significantly lower,and the IFN-γ and IL-10 level were significantly increased compared with the model group.The correlation analysis showed that the IFN-γ,IL-4,IL-17 and IL-10 were negatively related.It was concluded that H.officinalis L.played a certain role on correcting imbalances of Th1/Th2 and Th17/Treg.
出处
《世界科学技术-中医药现代化》
北大核心
2013年第3期591-594,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
新疆维吾尔自治区自然基金委面上项目(2009211A21):祛风止痉方治疗过敏性哮喘实验研究
负责人:李风森
关键词
慢性阻塞性肺病
酶联免疫吸附法
神香草
Chronic obstructive pulmonary disease
ELISA
Hyssopus officinalis L.
