膳食诱导肥胖小鼠下丘脑Kiss-1和GPR54 mRNA表达

Expressions of hypothalamic Kiss-1 and GPR54mRNA in diet-induced obese mice

目的探讨Kiss-1/G蛋白偶联受体54(GPR54)基因在肥胖导致雄性小鼠促性腺激素释放激素低下中作用。方法 6周龄C57bl/6雄性小鼠,共48只,动物随机分为2组:对照组12只,给予基础饲料,实验组36只,给予高脂饲料,饲养8周后,小鼠按体重分为肥胖倾向(DIO)和肥胖抵抗(DIO-R)2组,下腔静脉抽血后全部断头处死,称量生殖脏器、睾丸周和肾周脂肪含量,电镜检测睾丸间质细胞改变,迅速取出下丘脑组织,检测Kiss-1和GPR54 mRNA表达水平。结果与对照组比较,DIO组小鼠肾周脂肪、附睾脂肪[(0.59±0.10)、(1.16±0.26)g]及其系数[(0.018±0.002)、(0.036±0.007)]明显升高(P<0.05);DIO组小鼠睾丸系数(0.66±0.08)明显低于对照组(0.77±0.03)(P<0.05);DIO组小鼠下丘脑Kiss-1 mRNA表达水平(0.39±0.07)明显低于对照组(1.00±0.05)(P<0.05)。结论 Kiss-1 mRNA表达降低可能在雄性小鼠促性腺激素释放激素低下中起作用。

Objective To explore the role of Kiss-1/G protein-coupled receptor 54（GPR54） on the reductiion of gonadotropin-releasing hormone in obese male mice.Methods A total of 48 C57bl / 6 male mice（6 weeks） were selected and randomly divided into 2 groups：12 in control group with basal diet,36 in the experimental group with high fat diet.In 8 weeks,the mice were divided into diet-induced obese（DIO） and diet-induced obese resistant（DIO-R） group according to body weight of the mice.Reproductive organs,perirenal fat and testicular fat were weighted.The level of Kiss-1 and GPR54 mRNA were detected.Results The weight of perirenal fat and testicular fat（0.59±0.10 g and 1.16±0.26 g） and the ratio to weight（0.018±0.002 and 0.036±0.007）in DIO group were significantly lower than those of the control group（P＆lt;0.05 for all）.The level of Kiss-1 mRNA in the hypothalamus in DIO group（0.39±0.07）was significantly lower than that of the control group（1.00±0.05,P＆lt;0.05）.Conclusion The decreased expression of Kiss-1 mRNA may play a role in the low level of gonadotropin release hormone in obese male mice.