肽组学样品前处理方法与技术进展

Development of sample pretreatment approach and technology for peptidome

肽组学作为蛋白质组学研究领域的一个重要分支,在生物标志物发现、疾病早期诊断、生物制药等领域有着广阔的应用前景。在肽组学研究过程中,样品前处理是首要环节。本文对肽组学样品前处理方法与技术进行了综述,内容涉及超滤技术、有机溶剂沉淀方法、固相萃取技术等。样品中大量高丰度蛋白质的存在是肽组学发展面临的最大挑战,因此迫切需要发展快速、有效、高通量、自动化的样品前处理方法和技术。

As a branch of proteomics, peptidome has been extensively applied in biomarker discovery, early diagnosis and pharmacy. In the research of peptidome, sample pretreatment plays a vital role. Here, we review the pretreatment approaches and techniques of peptidome, mainly including ultrafiltration, organic solvent precipitation, solid phase extraction and so on. The biggest challenge of the development of peptidome is the large number of high-abundance proteins in the sample. Therefore, there is an urgent need to develop the fast, efficient, high- throughput and automated sample preparation methods and techniques.