硼酸亲和整体柱在富集中药活性物质中的应用

Enrichment of cis-diols active components in traditional Chinese medicines by boronate affinity monolithic column

以4-乙烯基苯硼酸(VPBA)为功能单体,季戊四醇三丙烯酸酯(PETA)为交联剂,乙二醇和二甘醇为二元致孔剂,通过热引发自由基聚合反应,在毛细管内原位聚合制备得到一种poly(VPBA-co-PETA)毛细管整体柱。所制备的整体柱具有典型的硼酸亲和特性,可以特异性地捕获含有顺二羟基的化合物。在前期实验的基础上,将其用于两种中草药蒲公英和刺果卫矛中含顺二羟基的小分子活性物质的富集。这两种中药提取物直接用高效液相色谱进行检测时,其活性成分绿原酸等因含量太低而使检测困难或无法检测;经过硼酸亲和整体柱富集后收集洗脱液进行检测,色谱峰响应则显著提高。该结果表明所制备的poly(VPBA-co-PETA)整体柱可以作为富集中药提取物中含顺二羟基活性物质的有效手段。

A novel poly（4-vinylphenylboronic acid （ VPBA）-co-pentaerythritol triacrylate （ PE- TA） ） monolithic column was prepared by thermal-initiated copolymerization of VPBA and PE- TA in a binary porogen system comprising diethylene glycol and ethylene glycol. The fabricated monolithic column demonstrated good boronate affinity property, which can specifically cap- ture cis-diols containing compounds. We developed such a monolithic column and applied it to enrich active components in Herba Taraxaci and Euonymus acanthocarpus. The extracts of the two traditional Chinese medicines were separated and enriched on μHPLC by the prepared monolith, and the fractions were collected and examined by HPLC. The results showed that the responses of the corresponding peaks were greatly improved, indicating that the poly （VPBA-co- PETA） monolithic column can be efficiently used to enrich active components in traditional Chinese medicines containing cis-diols group.