厌氧后猪场废水资源化制备微生物絮凝剂

Preparation of bioflocculant using anerobic treated piggery wastewater as substrate

通过检测碳源、氮源、磷酸盐和pH等对微生物絮凝剂絮凝率的影响,考察了红平红球菌利用厌氧处理后的猪场废水生产微生物絮凝剂的性能。通过正交实验研究各因素对絮凝率的交互影响以及影响程度的相对大小,从而确定了最佳培养基。结果表明,1.0 L厌氧处理后的猪场废水中加入蔗糖10.0 g、KH2PO42.0 g、K2HPO45.0 g、NaCl 1.0 g、Mg-SO40.2 g,在温度30℃、摇床速度120 r/min、pH=8的条件下发酵72 h后,发酵液的絮凝率可达到92.57%。表明厌氧处理后的猪场废水中加入适量的蔗糖和磷酸盐,完全可以作为一种有效的生产微生物絮凝剂的培养基。

Through studying the effects of carbon source, nitrogen source, phosphorus source and initial pH value onbioflocculant production, this research investigated the production of bioflocculant by Rhodococcus erythropolis using the piggery wastewater by anaerobic treatment as substrate. Meanwhile, through studying the effect of variety factors on the flocculation rate as well as the degree by orthogonal experiment, the optimized medium was ensured. The results showed that, when the composition of the piggery wastewater medium is as follows： piggery wastewater of 1.0 L, sucrose of 10 g/L, KH2PO4 of 2.0 g/L, K2HPO4 of 5.0 g/L, NaCl of 1.0 g/L, MgSO4 of 0.2 g/L, and after 72 hours of cultivation under the conditions of 30℃, 120 r/min and the medium pH of 8.0, the flocculating rate can reach 92.57%. This is concluded that the piggery wastewater can be used as an effective and low-cost substrate on bioflocculant production by adding the right amount of sucrose and phosphate.