IL-6对ADM诱导HL-60细胞凋亡影响的研究

Study of affection of apoptosis in HL-60 cell line induced by IL-6 and ADM agents

目的 :探讨白细胞介素 - 6 (IL - 6 )加阿霉素 (ADM )对早幼粒细胞株 (HL - 6 0 )凋亡作用的影响。方法 :采用DNA琼脂糖凝胶电泳 ,光镜形态学观察 ,台盼兰活细胞拒染试验分别检测IL - 6、ADM、及IL - 6加ADM对HL - 6 0细胞凋亡的影响。结果 :①中、高剂量IL- 6抑制细胞凋亡 (P <0 0 5 ) ,低剂量无影响 ,(P >0 0 5 )。②ADM诱导细胞凋亡的作用强度及细胞凋亡百分率依次为 :高剂量 >中剂量 >低剂量。③低剂量IL - 6加ADM组的细胞凋亡百分率与ADM组比较无明显差异 (P >0 0 5 ) ,中、高剂量IL - 6加ADM组的细胞凋亡百分率、梯状电泳带 (Ladder)低于同一时间对照组 (P <0 0 5、P <0 0 1)。结论 :IL - 6加ADM可抑制白血病细胞凋亡 ,提示在应用ADM药时慎用IL - 6。

Objective:Apoptosis in leukemic cells induced by IL-6 additing ADM is investigated.Methods:Three kinds of methods such as typanblue exclusion test, morphological study and DNA agrose gel electrophoresis were utilized to detect affection of apoptosis in the acute promyeloid leukemic cell line(HL-60).Results:①Middle,high concentration of IL-6 may suppress apoptosis,( P <0 05),low that was no diference( L >0 05).②ADM could induce apoptosis in HL-60 cells,but the intensity of effect was high-dosage>middle-dosage>low-dosage( P <0 05).③Apoptosis Percentage of low concentration of IL-6 additing ADM was not different to control group,( P >0 05),but Apoptosis Percentage of middle,high concentration IL-6 additing ADM and DNA electrophoesis numbers of those were less than only chemotherapeutic drug groups at the some time( P <0 05, P <0 01).Conclusion:IL-6+ADM may suppress apoptosis of leukemic cells. IL-6 can't be used or can be Careful used during ADM being used.