去氢雌马酚对急性早幼粒白血病NB4细胞的增殖抑制作用及其初步机制研究

Study on the Anti-proliferation Effect and Preliminary Mechanism of Dehydroequol on NB4 Cells

目的研究黄酮类化合物去氢雌马酚(dehydroequol)对急性早幼粒白血病NB4细胞的增殖抑制作用,并对其作用机制进行初步研究。方法应用噻唑蓝比色法及台盼蓝计数法观察去氢雌马酚及其结构类似物对不同肿瘤细胞增殖的影响,并应用细胞周期分析及细胞凋亡检测研究去氢雌马酚抑制NB4细胞增殖的机制。结果通过比较去氢雌马酚及其3个结构类似化合物对NB4细胞的增殖抑制作用。发现去氢雌马酚抗肿瘤活性最强,并且去氢雌马酚能显著抑制多种肿瘤细胞增殖,其中对急性早幼粒细胞白血病NB4细胞的抑制作用更显著,并能浓度与时间依赖性地抑制NB4细胞增殖,引起NB4细胞G1期阻滞并诱导细胞凋亡。结论去氢雌马酚对急性早幼粒白血病NB4细胞的增殖有很强的抑制作用,并且通过引起细胞G1期阻滞和诱导细胞凋亡而显著抑制NB4细胞的增殖。

Objective To study the anti-proliferation effect and preliminary mechanism of dehydroequol on NB4 cells. Methods Thiazole blue colorimetry and typan blue exclusion assay were used to study the effect of de- hydroequol and analogues on proliferation of several types of tumor cells. Cell cycle analysis and apoptosis assay were used to investigate the preliminary mechanism of dehydroequol on NB4 cells. Results Dehydroequol was the strongest of these compounds by comparmy dehydroequol and its analogs. Dehydroequol significantly inhibited the proliferation of many cancer cell lines, especially the proliferation of NB4 cells. Dehydroequol significantly inhibited the proliferation of NB4 cells in a close and time dependent manner, arrested NB4 cells in G1 phase and induced apoptosis in NB4 ceils. Conclusion Dehydroequol can exert a conspicuous anti-proliferation on NB4 cells possibly by arresting NB4 cells in G1 phase and inducing apoptosis in NB4 cells.