高效液相色谱法同时快速测定饮料中15种常用添加剂

Simultaneous determination of fifteen food additives in drink by high performance liquid chromatography

目的:建立饮料中安赛蜜、苯甲酸、山梨酸、糖精钠、柠檬黄、新红、苋菜红、靛蓝、胭脂红、咖啡因、日落黄、诱惑红、亮蓝、酸性红(偶氮玉红)、赤藓红等15种常用添加剂同时测定的高效液相色谱方法。方法:样品中15种添加剂以甲醇-0.02 mol/L乙酸铵溶液为流动相,梯度洗脱,C18柱分离,二级管阵列检测器检测。结果:方法线性范围在1.0 mg/L～50 mg/L,最低检出限为0.12 mg/L～0.30 mg/L,相对标准偏差为0.7%～2.8%,回收率为93.0%～115.0%。结论:该方法简便、快速、准确,重复性好,可用于饮料中15种常用添加剂的测定。

Objective： To establish a high performance liquid chromatographic method for the determination of fifteen food additives, including acesulfame - K, benzoic acid, sorbic acid, saccharin sodium, tartrazine, new red, amaranth, indigotic, ponceau 4R, caffeine, sunset yellow, allure red, brilliant blue, azorubin, erythrosine in drink. Methods： Fifteen food additives were separated on C18 column by gradient elution using CH3OH -0. 02mol/L NH4AC as mobile phase, and detected with a diode - array detector. Results： The linear range of the method was 1.0 - 50 mg/L. The limits of detection were 0.12 mg/L - 0.30 mg/L. The recoveries were 93.0% - 115.0%, and the relative standard deviation were 0.7% - 2.8%. Conclusion： The method was simple, rapid, accurate and repeatable, so it was suitable for the determination of fifteen food additives in drink.