微波消解-氢化物发生原子荧光光谱法测定生物材料中砷汞

Determination of arsenic and mercury in biological materials by atomic fluorescence spectrometry with microwave digestion

目的:建立微波消解-氢化物发生原子荧光光谱快速测定生物材料中砷汞的方法。方法:人发、尿样、血样经微波消解后,用氢化物发生原子荧光光谱测定砷、汞的浓度。结果:砷、汞浓度在0.021μg/L～20.0μg/L、0.0072μg/L～2.00μg/L范围具有良好的线性,相关系数为0.9997和0.9998,检出限分别为0.021μg/L、0.0072μg/L,相对标准偏差分别为0.80%～1.07%、0.82%～1.45%。加标回收率分别为92.5%～103.0%和94.0%～101.5%。结论:该法简便快速、灵敏度高、重现性好,适用于生物材料中砷、汞的快速测定。

Objective： To establish a method for determination of arsenic （As） and mercury （Hg） in biological material by atomic fluorescence spectrometry （AFS）using microwave digestion. Methods： The biological materials were digested using microwave digestion and then As and Hg were detected with AFS. Results： The method had good linearity for As in the range of 0. 021 ug/L to 20.0 ug/L with r = 0. 9997 and for Hg in the range of 0. 0072 ug/L - 2.00 ug/L with r = 0.9998. The detection limits were 0.021 ug/L and 0. 0072 ug/L for As and Hg, respectively, with the recoveries of 92.5% - 103.0% and 94.0% - 101.5%. The RSDs of arsenic and mereucy were 0.80% -1.07% , and 0.82% -1.45% respectively. Conclusion： This method is simple and rapid with good accuracy and reproducibility. So it is suitable for the determination of As and Hg in biological materials.