广州市泔水油的原料中优势菌种鉴定及其检测靶标的确定

Identification of dominant bacteria and testing target in swill oil materials in Guangzhou

目的:本研究想寻找泔水油的特有靶标,为下一步研制快速检测泔水油试剂奠定基础。方法:本实验对泔水样品采用传统方法进行细菌培养、分纯和生化鉴定细菌种类,然后计数每份样品各典型菌落数量,确定出优势菌种;再用鲎试剂凝胶法对比正常食用油和泔水油里面的脂多糖含量。结果:30份样品,检出大肠埃希菌26株,肺炎克雷伯菌肺炎亚种15株,在单个样品中这两种菌的菌落数量也比其它菌多。鲎试验对比中发现:泔水油中细菌代谢产物--脂多糖含量比正常食用油的高出100-1000倍。结论:确定泔水中的优势菌种为肺炎克雷伯肺炎亚种和大肠埃希菌。细菌代谢产物——脂多糖可以作为一项泔水油的抗原检测靶标。

Objective： To define a new marker which would be regarded as antigen to prepare antibody for laying a solid foundation for further researcher on rapid detection of swill oil. Methods ： Samples of swill oil were cultivated in selective medium. Then the typical colonies were selected to cultivate independently and examined by biochemical identification. And typical bacterial colonies were counted on each sample to identify the dominant bacterial. Besides, the LPS was examined by contrasting good oil to swill oil with tachypleus amebocyte lysate（TAL）. Results： In thirty tested samples, there were 26 strains of Escherichia coli （E. coli） and 15 strains of Klebsiella pneumoniae ssp pneumoniae （KP）, and their bacterial colonies were more than others in single sample. And the quantity of lipolpolysaccharide （LPS） of swill sample was 100 to 1000 folds more than it in edible oil. Conclusion： Based on these study mentioned above, it can be proved that KP and E. coli are dominant bacteria. LPS is the marker of these bacteria metabolin in swill oil.