常州市一起急性胃肠炎的病原学研究

Pathology research on an acute gastroenteritis outbreak in Changzhou

目的:明确一起急性胃肠炎疫情的病原体及其基因型。方法:对36份疑似病例标本采用荧光定量PCR方法进行诺如病毒核酸检测,选取8份阳性标本进行RT-PCR扩增,扩增产物经序列测定分析诺如病毒的基因亚型并进行系统进化分析。结果:荧光定量PCR检测出15份阳性标本,均为诺如病毒GII型。对其中的8份标本进行核酸序列测定,确定均为GII/12型诺如病毒。结论:本次急性胃肠炎疫情由诺如病毒GII/12型引起。

Objective： To determine the pathogen and genogroup of an acute gastroenteritis outbreak by laboratory diagnosis of suspected eases samples. Methods： Thirty - six samples were tested for Norovirus by Real - time PCR. Eight positive samples were amplified by reverse transcriotion PCR, and the products were sequenced, then genotype was identified and phylogenetic tree was constructed. Results： Norovirus GII was detected positively in 15 of 36 samples. Sequencing and phylogenetic analysis revealed that 8 strains of Norovirus belonged to GII/12. Conclusion： The epidemic event of acute gastroenteritis was caused by GII/12 Norovirus.