念珠菌显色培养基检测效果初步评价

Primary evaluation of chromogenic media in detection of Candida

目的:初步评价显色培养基对念珠菌的检测效果。方法:本实验室研制的念珠菌显色培养基HKM-CC,与国外科玛嘉同类产品CHROMagarCC及传统培养基沙保氏琼脂(SDA),对相关菌株以及临床分离株进行对比测试。结果:HKMCC与CHROMagarCC均具有较好的特异性,优于SDA。HKMCC与CHROMagar-CC显色培养基均具有较好的选择性,HKMCC对非目标菌的抑制率比CHROMagarCC高9%,比传统培养基SDA高71.2%。8株念珠菌在三种培养基上的生长率均大于0.8。经统计分析,试验的三种培养基生长率差异不显著(P>0.05)。结论:利用显色培养基检测念珠菌,特别是白色念珠菌,具有较好的检测效果。

Objective：To evaluate the efficacy of chromogenic medium for Candida detection. Methods： A new Candida chromogenic medium HKMCC was compared with CHROMagar CC and Sabouraud dextrose agar （SDA） for detection of Candida on reference strains and clinical isolated strains. Results：The specificity of HKMCC was as same as that of CHROMagar CC, and better than that of SDA. The selectivity of HKMCC was 9% higher than that of CHROMagar CC, and 71.2% higher than that of SDA for non target bacteria inhibition. The growth rate of 8 strains on three culture media were all above O. 8. Based on the statistical analysis, there are no significant difference among three culture media （P 〉 0.05） in this study. Conclusion： Chromogenic Candida medium has a good effect in detecting Candida, particularly C. albicans.