三种创伤弧菌分离培养基的性能测试研究

Study on performance test for three kinds of vibrio vulnificus isolation media

目的:对不同厂家的改良纤维二糖-多粘菌素B-多粘菌素E培养基(mCPC)、纤维二糖-多粘菌素E培养基(CC)和硫代硫酸盐-柠檬酸盐-胆盐-蔗糖培养基(TCBS)进行性能测试。方法:将试验样品按不同剂量人工染菌后,接种于3%氯化钠碱性蛋白胨水(N-APW)增菌,(35±2)℃培养24 h后再划线分离于不同厂家的mCPC、CC、TCBS平板中,比较其阳性检出率。选用7株创伤弧菌标准株和分离株分别测试其在上述培养基上的生长率。结果:mCPC、CC对人工染菌试样中创伤弧菌的阳性分离率高于TCBS;试验菌株在mCPC、CC上的生长率明显高于TCBS,HK mCPC、CC培养基上的生长率均高于厂家2。结论:对海产品中创伤弧菌的分离效果,mCPC、CC培养基优于TCBS。HKM厂家的mCPC、CC培养基均优于厂家2。

Objective：To test the performance of modified cellobiose - polymixin B - colistin （mCPC） plate, cellobiose- colistin （CC） plate and thiosulphate citrate bile source （TCBS） plate from different manufacturers. Methods： The samples were artificially contaminated with different doses, then inoculated to 3% NaCl alkaline peptone water （ N - APW） at （35 ± 2） ℃ for 24 h, then isolated at mCPC, CC, TCBS plates from different manufacturers using plate streaking method, the positive detection rates were compared. The growth rates of seven Vibrio vulnificus strains were tested on these plates. Results： The positive detection rates of artificially contaminated V. vulnificus samples using the mCPC and CC plates were higher than that of TCBS. The growth rates of mCPC, CC were also higher than that of TCBS, and mCPC and CC plates of HKM were higher than manufacturer 2 for seven strains. Conclusions： The separation effect of V. vulnificus samples in marine was found that mCPC and CC plate were superior to TCBS. HKM mCPC and CC were better than manufacturer 2.