珠江水霍乱弧菌检测方法探讨

Discussion on detection method for Vibrio cholera in Pearl river water

目的:比较三种霍乱弧菌的检测方法,提高珠江水霍乱弧菌的检出率。方法:用荧光PCR法、胶体金法、常规培养法同时对珠江水中霍乱弧菌进行检测。结果:实时荧光PCR法共检出霍乱弧菌核酸阳性15份,胶体金法检出13份;常规细菌培养法检出11份。实时荧光法灵敏度=90.9%;特异度=98.9%;约登指数=89.8%;Kappa指数=0.81。胶体金法灵敏度=72.7%;特异度=99.4%;约登指数=72.1%;Kappa指数=0.78。结论:实时荧光PCR法与常规细菌培养法结果、胶体金法与常规细菌培养法结果比较差异具有统计学意义,可以考虑用实时荧光PCR法对大量样本进行初筛,再与分离培养法联合使用,确保霍乱病例的早发现、早诊断,其联合使用值得在基层卫生系统防治工作中推广和使用。

Objective： To compare the three detection methods to improve detection rate of Vibrio cholera in Pearl River water. Methods： Fluorescent PCR method, colloidal gold method, conventional culture method were used for detection of Vibrio cholera in Pearl River water. Results： With real - time fluorescent PCR method, fifteen Vibrio cholera strains were found positive, the sensitivity was 90.9%, the specificity was 98.9%, and the Kappa value was 0.81 ; With colloidal gold method, thirteen Vibrio cholera strains were found positive, the sensitivity was 72. 7%, the specificity was 99.4%, and the Kappa value was 0.78; With conventional bacterial culture method, eleven Vibrio cholera strains were found positive. Conclusion： The detection results of real - time fluorescent PCR method, colloidal gold method and conventional bacterial culture method had statistical difference. Real - time fluorescent PCR method is suitable for preliminary screening of large number of samples. Combination of real - time fluorescent PCR and culture method contribute to the early finding and diagnosis of cholera cases, worthy to popularize and apply in basic hygiene system.