GenoType MTBDRplus从涂阳痰标本中检测结核分枝杆菌耐药性的效果评价

Evaluation of GenoType MTBDRplus for drug-resistance detection of Mycobacterium tuberculosis from smear-positive sputum specimens

目的:评价耐多种药物结核病快速诊断方法(GenoType MTBDRplus)在涂阳痰标本中检测结核分枝杆菌利福平和异烟肼耐药性的效果。方法:对纳入的涂阳痰标本同步进行L-J分离培养、比例法药物敏感性试验和GenoType MTBDRplus检测,以比例法药物敏感性试验结果为判定标准,分析该方法的灵敏度和特异度以及两种方法的一致性。结果:GenoType MTBDRplus检测利福平、异烟肼敏感度分别为90.6%和82.7%,特异度分别为98.7%和97.3%。结论:在涂阳痰标本中应用GenoType MTBDRplus检测结核分枝杆菌利福平和异烟肼耐药性是一种快速、灵敏、特异度高的诊断方法,可在有条件的地区推广使用。

Objective： To evaluate the effect of GenoType MTBDRplus assay for rifampin （RIF） and isoniazid （INH） susceptibility testing on mycobaeterium tuberculosis from sputum specimens. Methods： Conventional drug susceptibility testing and GenoType MTBDRplus assay were used to detect resistance to RIF and INH of smear - positive sputum specimens. The sensitivity and specificity of GenoType MTBDRplus assay were analyzed. Results： The sensitivities of GenoType MTBDRplus assay for detecting RIF and INH were 90.6% and 82.7% , respectively. Their specificities were 98.7% and 97.3% , respectively. Conclusion： GenoType MTBDRplus assay is a rapid method with high sensitivity and specificity for detecting the RIF and INH resistance in smear - positive sputum specimens and should be promoted appropriately.