神经胶质生长因子促进受损周围神经功能恢复

Glial growth factor accelerate functional recovers of injured peripheral nerve

目的评价了重组体人类神经胶质生长因子Ⅱ（ｒｈＣＧＦ２）对大鼠坐骨神经挤压伤后运动功能恢复的效果。方法７３只大白鼠被分为 ３组。一组（ｎ＝ ５）实施模拟挤压操作。在二组（ｎ＝ ３４）、三组（ｎ＝ ３４）中，每只动物选一 ５ ｍｍ长的坐骨神经段承受 １００ ｇ挤压２ ｈ。其中，三组用 ｒｈＧＧＦ２治疗（１ｍｇ／ｋｇ挤压后皮下注射，１次／ｄ，连续４ ｄ），二组用相同量的载体治疗。通过计算坐骨神经功能指数（ＳＦＩ）和测量趾长伸肌（ＥＤＬ）强直收缩力来评价神经运动功能恢复。结果与二组相比，三组神经在术后 １１－２１ｄ表现出显著的 ＳＦＩ改善。三组肌肉收缩力强于二组，并从４— １４ ｄ在 ７０ Ｈｚ和 １００ Ｈｚ刺激下有显著性差异。组织学切片显示三组呈现较轻的轴突演变和较早的神经再生。结论用ｒｈＧＧＦ２治疗坐骨神经挤压伤能有效的促进神经再生，进而显著改善其功能的恢复。

Objective To evaluate effect of the recombinant human glial growth factor II(rhGGF2), on the recovery of motor function of rat sciatic nerve following crush injury. Methods 73 rats were divided into three groups. A sham operation was performed in group I(n = 5 ). A 5 - mm segment of the sciatic nerve was subjected to a 100 g crush load for 2 hours of duration in groups II(n = 34)and III(n = 34). Group III was treated with rhGGF2. and group II was given the same volume of vehicle. Motor functional recovery was assessed by calculating the sciatic functional index (SFI) and measuring tetanic contractile force of the extensor digitorum longus(EDL). Reults Group III exhibited a significant improvement of the SFI on postoperative days from 11 to 21 when compared to Group II. The EDL contractile force was generally stronger in group III than in group II, with a significant difference at 7O and 100 Hz stimulus from day 4 to day 14. Histological sections revealed less axonal degeneration and earlier regeneration of nerve fibers in group III. Conclusion It suggests that treatment with rhGGF2 is effective in promoting nerve regeneration and, as a result, can significantly improve the functional recovery of rat sciatic nerve following crush injury.