独蒜兰的化学成分研究

Chemical constituents from Pleione bulbocodioides

目的对独蒜兰Pleione bulbocodioides假鳞茎的化学成分进行研究。方法采用正、反相硅胶柱色谱,凝胶柱色谱及制备高效液相色谱等方法对独蒜兰乙醇提取物的醋酸乙酯部位进行分离纯化,根据波谱数据结合理化性质鉴定化合物结构。结果从独蒜兰乙醇提取物的醋酸乙酯部位分离得到13个化合物,分别鉴定为4,4′-二羟基二苯基甲烷(1)、phillygenin(2)、表松脂醇(3)、2,5,2′,5′-四羟基-3-甲氧基联苄(4)、大黄酚(5)、大黄素甲醚(6)、汉黄芩素(7)、β-谷甾醇(8)、山药素Ⅲ(9)、对羟基苯甲酸(10)、独蒜兰西醇F(11)、3,3′-dihydroxy-2-(4-hydroxybenzyl)-5-methoxybibenzyl(12)、3′,5-dihydroxy-2-(4-hydroxybenzyl)-3-methoxybibenzyl(13)。结论化合物1～8为首次从独蒜兰属植物中分离得到。

Objective To study the chemical constituents in the pseudobulbs of Pleione bulbocodioides.Methods The chemical constituents from the EtOAc fraction of ethanol extract from pseudobulbs in P.bulbocodioides were isolated and purified by various chromatographic methods,including silica gel,ODS,and Sephadex LH-20 columns,and semi-preparation HPLC.Structures of the isolated compounds were elucidated based on NMR,and MS data,as well as by comparison with the previous data.Results Thirteen compounds were obtained from the EtOAc fraction of ethanol extract and identified as 4,4′-dihydroxy-bisphenxyl（1）,phillygenin（2）,（＋）-epipinoresinol（3）,2,5,2′,5′-tetrahydroxy-3-methoxybibenzyl（4）,chrysophanol（5）,physcion（6）,5,7-dihydroxy-8methoxyflavone（7）,β-sitosterol（8）,batatasin Ⅲ（9）,p-hydroxybenzoic acid（10）,shanciol F（11）,3,3′-dihydroxy-2（4-hydroxybenzyl）-5-methoxybibenzyl（12）,and 3′,5-dihydroxy-2-（4-hydroxybenzyl）-3-methoxybibenzyl（13）.Conclusion Compounds 1—8 are isolated from the plants in genus Pleione D.Don for the first time.