泡核桃壳的化学成分研究

Study on chemical constituents in shells of Juglans sigillata

目的研究泡核桃Juglans sigillata壳的化学成分。方法采用硅胶、反相RP18、Sephadex LH-20、MCI等色谱柱以及半制备HPLC柱及制备薄层等方法对泡核桃壳的化学成分进行分离纯化,根据理化性质与波谱数据对所分离到的化合物进行结构鉴定。结果从泡核桃壳95%乙醇提取物中共分离鉴定15个化合物,其中包括7个酚苷类化合物:它乔糖苷(1)、牡丹酚苷A(2)、4-O-β-D-glucopyranosylvanillc acid(3)、breynioside A(4)、1-O-香草酰-β-D-葡萄糖苷(5)、6′-O-vanilloyltachioside(6)、6′-O-vanilloylisotachioside(7);3个苯丙酸类化合物:6-O-feruloyl-D-glucopyranose(8)、methyl-4-O-coumaroylquinate(9)、5-p-cis-coumaroylquinic acid(10);2个萘酮类化合物:胡桃苷A(11)、胡桃苷E(12);1个降倍半萜苷类化合物:长春花糖苷(13);1个黄酮类化合物:二氢槲皮素(14);1个脱落酸衍生物:二氢红花菜豆酸-4′-O-β-D-吡喃葡萄糖苷(15)。结论除化合物14外,其他化合物均为首次从该植物中分离得到,其中化合物1～4、13、15为首次从该属植物中分离得到。

Objective To investigate the chemical constituents in the shells of Juglans sigillata.Methods The chemical constituents were isolated by silica gel,RP18,Sephadex LH-20,and MCI column chromatography and semi-preparative HPLC and so on.The structures were identified on the basis of spectroscopic analysis and chemical evidence.Results Fifteen compounds were isolated and identified in the 70% ethanol extract from the shells of J.sigillata including seven phenolic glycosides： tachioside（1）,mudanoside A（2）,4-O-β-D-glucopyranosylvanillc acid（3）,breynioside A（4）,1-O-vanilloyl-β-D-glucose（5）,6′-O-vanilloyltachioside（6）,and 6′-O-vanilloylisotachioside（7）;three phenylpropanoide acid glycosides： 6-O-feruloyl-D-glucopyranose（8）,methyl-4-Ocoumaroylquinate（9）,and 5-p-cis-coumaroylquinic acid（10）;two tetralone glycosides： juglanin A（11） and juglanin E（12）;one norsesquiterpenes glycoside： roseoside（13）;one flavone： toxifolin（14）;and one glucosylated abscisic acid derivate：（1′R,3′R,5′R,8′S）-epi-dihydrophaseic acid β-D-glucoside（15）.Conclusion Except compound 14,the other compounds are isolated from the shells of J.sigillata for the first time.And compounds 1—4,13,and 15 are reported for the first time from the plants in genus of Juglans L.