维药小新塔花和芳香新塔花的HPLC指纹图谱研究

HPLC fingerprint analysis on Wei Medicine Ziziphora tenuior and Ziziphora clinopodioides

目的建立小新塔花和芳香新塔花的HPLC指纹图谱分析方法,并同时测定8批小新塔花和22批芳香新塔花中金丝桃苷、槲皮素、胡薄荷酮的量。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.08%甲酸水溶液进行梯度洗脱,同时测定了小新塔花和芳香新塔花中金丝桃苷、槲皮素、胡薄荷酮的量,建立了不同产地8批小新塔花和22批芳香新塔花的指纹图谱。结果根据主成分分析、聚类分析,将30批药材分为2类,建立了2种新塔花的HPLC指纹图谱。不同产地的2种新塔花中金丝桃苷、槲皮素和胡薄荷酮等成分的量存在较大差异;芳香新塔花不含槲皮素,而小新塔花中含有;芳香新塔花中的金丝桃苷和胡薄荷酮量高于小新塔花;小新塔花中金丝桃苷量为49.72～204.33μg/g,槲皮素量为161.06～213.22μg/g,胡薄荷酮量为36.19～93.29μg/g;芳香新塔花中金丝桃苷量为174.15～802.24μg/g,胡薄荷酮量为77.43～353.45μg/g。结论不同品种的新塔花指纹图谱存在一定的差异,可为新塔花药材的质量控制、品种鉴定及划分提供一定的参考依据。

Objective To establish HPLC fingerprint for the analysis on Ziziphora tenuior and Z.clinopodioides and the determination of hyperin,rutine,and pulegone in eight batches of Z.tenuior and 22 batches of Z.clinopodioides synchronously.Methods The HPLC method was used with Kromasil C18 column（250 mm × 4.6 mm,5 μm）,and a mixture of methanol-0.08 % HCOOH was used as mobile phase with gradient elution.The HPLC fingerprint for eight batches of Z.tenuior and 22 batches of Z.clinopodioides was established.Results The HPLC fingerprints of the two species were obviously different,so two modes were established for two species,respectively.The eight batches of Z.tenuior and 22 batches of Z.clinopodioides were classified into two groups based on the result of principal component,hierarchical cluster,and similarity analyses.Hyperin and pulegone were the common compositions;quercetin（161.06—213.22 μg/g） was founded in Z.tenuior,but not in Z.clinopodioides;the contents of hyperin（174.15—802.24 μg/g）,pulegone（77.43—353.45 μg/g） of Z.clinopodioides were higher than those of Z.tenuior（49.72— 204.33 and 36.19—93.29 μg/g）.Conclusion Z.tenuior and Z.clinopodioides have the different fingerprint,which could provide the evidence for their quality control,species identification,and classification.