摘要
OBJECTIVE:To study the possible roles of Jinlong capsule(JLC) on the proliferation and apoptosis of human pancreatic cancer cells BxPC-3.METHODS:The human pancreatic cancer cells BxPC-3 were treated with JLC at the concentration of 0.05-1.00 mg/mL for 24-120 h.The inhibition rate of JLC on human pancreatic cancer cells BxPC-3 was detected by 3-(4,5-dimethiylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay.Flow cytometry was employed to measure cell apoptosis using Annexin V-FITC/Propidium iodide(AV-FITC/PI) method.Cell cycles were determined by PI staining.The expression of S100 Calcium binding protein A4(S100A4) in cell matrix was measured by enzyme-linked immunosorbent assay(ELISA).The expression levels of apoptosis-related protein such as BCL2/adenovirus E1B 19 kDa interacting protein 3(BNIP3),B-cell lymphoma/leukemia-2(Bcl-2) and Cys-teinylaspartate specific proteinase 3(Caspase-3) were detected byWestern blotting.RESULTS:JLC significantly inhibited the proliferation of human pancreatic cancer cells BxPC-3 in a dose-dependent and time-dependent manner.JLC promoted cell apoptosis and maintained cell cycle in S and G 2 /M phase rather than G 1 /G 0 phase.The expression of S100A4 in the cell matrix was reduced.The expression of cell apoptotic protein BNIP3 was increased while Bcl-2 was decreased.CONCLUSION:JLC can inhibit the proliferation of human pancreatic cancer cells BxPC-3 by stimulating cell apoptosis,arresting the cell cycle at S and G 2 /M phase which blocks the circulation of normal cell cycle and reducing the expression of S100A4 protein.Higher pro-apoptosis protein BNIP3 and lower anti-apoptosis protein Bcl-2 levels were found,which may be related to the apoptotic effects of JLC.
OBJECTIVE： To study the possible roles of Jinlong capsule （JLC） on the proliferation and apoptosis of human pancreatic cancer cells BxPC-3. METHODS： The human pancreatic cancer cells Bx- PC-3 were treated with JLC at the concentration of 0.05-1.00 mg/mL for 24-120 h. The inhibition rate of JLC on human pancreatic cancer cells BxPC-3 was detected by 3-（4,5-dimethiylthiazol-2-yl）-2, 5-diphe- nyl tetrazolium bromide （MTT） assay. Flow cytome- try was employed to measure cell apoptosis using Annexin V-FITC/Propidium iodide （AV-FITC/PI） method. Cell cycles were determined by PI staining. The expression of S100 Calcium binding protein A4 （S100A4） in cell matrix was measured by en- zyme-linked immunosorbent assay （ELISA）. The ex- pression levels of apoptosis-related protein such as BCL2/adenovirus E1B 19 kDa interacting protein 3 （BNIP3）, B-cell lymphoma/leukemia-2 （Bcl-2） andCys-teinylaspartate specific proteinase 3 （Cas- pase-3） were detected by Western blotting. RESULTS： JLC significantly inhibited the prolifera- tion of human pancreatic cancer cells BxPC-3 in a dose-dependent and time-dependent manner. JLC promoted cell apoptosis and maintained cell cycle in S and G2/M phase rather than G1/G0 phase. The expression of S100A4 in the cell matrix was re- duced. The expression of cell apoptotic protein BNIP3 was increased while Bcl-2 was decreased. CONCLUSION： JLC can inhibit the proliferation of human pancreatic cancer cells BxPC-3 by stimulat- ing cell apoptosis, arresting the cell cycle at S and G2/M phase which blocks the circulation of normal cell cycle and reducing the expression of S100A4 protein. Higher pro-apoptosis protein BNIP3 and lower anti-apoptosis protein Bcl-2 levels were found, which may be related to the apoptotic ef- fects of JLC.
