摘要
OBJECTIVE:To evaluate the effect of fermented extract of Kushen(Radix Sophorae Flavescentis) or non-fermented ESF on laryngeal neoplasms Hep2 cells.METHODS:Use 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay to explore the effect of fermented ESF and non-fermented ESF on Hep2 cells,and detect the mRNA and protein expression level of Bcl-2,Bax and Caspase-3 with reverse transcription polymerase chain reaction(RT-PCR) andWestern blot.RESULTS:Both fermented ESF and non-fermented ESF could inhibit laryngeal neoplasm's Hep2 cells,but and the cells did not response to the dilution 1:320 of fermented ESF,nor to the 1:1280 dilution of non-fermented ESF.As time progressed,the dilution 1:80 of fermented ESF and 1:320 dilution of non-fermented ESF could significantly reduce Bcl-2 mRNA and protein expression and down-regulate Caspase-3 mRNA and protein expression.Bax mRNA and protein were not expressed in Hep2 cells.CONCLUSIONS:Both fermented ESF and non-fermented ESF could inhibit the proliferation of Hep2 cells,and the effect of non-fermented ESF was significantly better than that of the fermented.
OBJECTIVE： To evaluate the effect of fermented ex- tract of Kushen （Radix Sophorae Flavescentis） or non-fermented ESF on laryngeal neoplasms Hep2 cells. METHODS： Use 3-（4,5-dimethylthiazol-2-yl）-2,5-di- phenyltetrazolium bromide （MTT） assay to explore the effect of fermented ESF and non-fermented ESF on Hep2 cells, and detect the mRNA and protein ex- pression level of Bcl-2, Bax and Caspase-3 with re- verse transcription polymerase chain reaction （RT-PCR） and Western blot. RESULTS： Both fermented ESF and non-fermented ESF could inhibit laryngeal neoplasm＇s Hep2 cells,but and the cells did not response to the dilution 1 ： 320 of fermented ESF, nor to the 1 ： 1280 dilution of non-fermented ESF. As time progressed, the dilu- tion 1 ： 80 of fermented ESF and 1 ： 320 dilution of non-fermented ESF could significantly reduce Bcl-2 mRNA and protein expression and down-regulate Caspase-3 mRNA and protein expression. Bax mRNA and protein were not expressed in Hep2 cells. CONCLUSIONS： Both fermented ESF and non-fer- mented ESF could inhibit the proliferation of Hep2 cells, and the effect of non-fermented ESF was sig- nificantly better than that of the fermented.
基金
Supported by the National Natural Science Foundation Project of China (No. 81274082)
the Research Project for Nonprofit Industry of State Administration of TCM (No.201007012-2-11)
