摘要
OBJECTIVE: To investigate the underlying mechanism of reduced myocardial ischemia-reperfusion (I/R) injury in rats using the traditionalTibetan medicine Sanweitanxiang powder (SWTX). METHODS: Rats were randomly divided into six groups (n=10) as follows: (a) propranolol dinitrate control group, given propranolol dinitrate 0.02 g/kg for 10 days before I/R, (b) SWTX with a high dose group, given SWTX 1.5 g/kg for 10 days before I/R, (c) SWTX with a medium dose group, given SWTX 1.25 g/kg for 10 days before I/R, (d) sham group (Sham), in which the rat heart was exposed by pericardiotomy but without I/R, (e) SWTX with a low dose group, given SWTX 1.0 g/kg for 10 days before I/R, and (f) I/R injury group. Rats were intragastrically pretreated with propranolol dinitrate orSWTX. After that, the operation to cause ischemia and reperfusion was conducted.The histopathologic changes of rat hearts were observed by hematoxylin and eosin staining and transmission electron microscopy. Ca2+ homeostasis protein expression was determined by western blot. RESULTS: After SWTX pretreatment, the development of ultrastructural pathological changes from IR injury was attenuated. A decrease in the expression of B-cell lymphoma 2 associated X protein, and an increase in the expression of B-cell lymphoma 2 were observed. An increased activation of extracellular signal regulated kinases were found. Compared with the sham group, the expression of sarcoplasmic reticulum calcium-ATPase, phospholamban, and calsequestrin were all up-regulated after pretreatment with SWTX. CONCLUSION: The protective mechanism of SWTX pretreatment on myocardial I/R injury might be related to its effect on maintaining the balance of calcium homeostasis in rat heart.
OBJECTIVE： To investigate the underlying mecha- nism of reduced myocardial ischemia-reperfusion （I/R） injury in rats using the traditional Tibetan med- icine Sanweitanxiang powder （SWTX）. METHODS： Rats were randomly divided into six groups （n=10） as follows： （a） propranolol dinitrate control group, given propranolol dinitrate 0.02 g/kg for 10 days before I/R, （b） SWTX with a high dose group, given SWTX 1.5 g/kg for 10 days before I/R, （c） SWTX with a medium dose group, given SWTX 1.25 g/kg for 10 days before I/R, （d） sham group （Sham）, in which the rat heart was exposed by peri- cardiotomy but without I/R, （e） SWTX with a low dose group, given SWTX 1.0 g/kg for 10 days be- fore I/R, and （f） I/R injury group. Rats were intragas- trically pretreated with propranolol dinitrate orSWTX. After that, the operation to cause ischemia and reperfusion was conducted. The histopatholog- ic changes of rat hearts were observed by hematox- ylin and eosin staining and transmission electron microscopy. Ca2. homeostasis protein expression was determined by western blot. RESULTS： After SWTX pretreatment, the develop- ment of ultrastructural pathological changes from IR injury was attenuated. A decrease in the expres- sion of B-cell lymphoma 2 associated X protein, and an increase in the expression of B-cell lympho- ma 2 were observed. An increased activation of ex- tracellular signal regulated kinases were found. Compared with the sham group, the expression of sarcoplasmic reticulum calcium-ATPase, phosphol- amban, and calsequestrin were all up-regulated af- ter pretreatment with SWTX. CONCLUSION= The protective mechanism of SWTX pretreatment on myocardial I/R injury might be re- lated to its effect on maintaining the balance of cal- cium homeostasis in rat heart.
基金
Supported by a Grant from the National Natural Sciences Foundation of China(No.81060368)
