摘要
目的探讨黄芪总黄酮（TFA）对柯萨奇B3病毒性心肌炎（VMC）小鼠急性期心肌组织连接蛋白43（Cx43）表达的影响。方法Balb／c小鼠腹腔注射柯萨奇B3病毒感染建立VMC小鼠模型，给予TFA40mg／L灌胃治疗，第14天无痛苦处死全部小鼠并留取心脏。免疫组织化学法检测Cx43蛋白水平表达，并进行定量分析。结果①VMC组小鼠心室肌组织炎症病灶中变性、坏死周围心肌细胞Cx43表达明显减弱，甚至阴性，分布不规则，Cx43蛋白表达明显低于对照组，差异有统计学意义（P〈0．01）。@TFA组Cx43蛋白表达明显高于模型组，差异有统计学意义（P〈0．01）。结论VMC心肌炎小鼠急性期心肌组织Cx43蛋白表达下降。TFA能明显提高CVB心肌炎小鼠急性期心肌组织Cx43蛋白表达。
Objective To study the effects of total flavonoids of astragalus （TFA） on the expressions of myocardial connexin43 in a murine model of acute viral myocarditis induced by B3 Coxsackie Virus and investigate the mechanism of the effects of TFA on arrhythmia induced by viral myocarditis. Methods Balb/c intraperitoneal injection of B3 coxsackie virus was used to establish VMC model. TFA 40 mg/L were given for irrigation. On the 14th day, ventricular myocardium was obtained. The expression of Connexin43 was determined by immunohistochemistry. Results （1）Compared with control group, Connexin43 were decreased remarkably in viral myocarditis and the decreased expression of connexin43 was located in the degenerative part of the myocardium （P〈0.01）. （2） The expression of connexin43 of the TFA treatment group were more higher than that of the model group, the difference was statistically significant （P〈0.01）. Conclusion The above results suggest that the expression of Cx43 decrease remarkably and TFA effectively increase the expression of Cx43 in a murine model of acute viral myocarditis induced bv B3 Coxsackie Virus.
出处
《中国心血管病研究》
CAS
2013年第7期546-548,I0004,共4页
Chinese Journal of Cardiovascular Research
