精氨酸对肠粘膜屏障功能的影响

Effects of Arginine on the Function of Intestinal Barrier

目的 :了解精氨酸对肠粘膜屏障功能的影响。方法 :雄性 SD大鼠 30只 ,随机分为三组。精氨酸组予精氨酸0 .2 5 g腹腔注射 ,假手术组和肠梗阻组予生理盐水作对照 ,1次 / d,共 5 d。再手术将肠梗阻组、精氨酸组大鼠结扎回肠 ,造成单纯性机械性肠梗阻模型 ,假手术组仅作剖腹探查。术后 2 4h处死大鼠采集标本。结果 :1细菌移位率和移位数水平肠梗阻组和假手术组相比明显升高 ,精氨酸组和肠梗阻组相比明显减少。 2血浆内毒素水平肠梗阻组明显高于假手术组 ,精氨酸组与肠梗阻组相比下降。 3病理结果显示假手术组肠粘膜基本正常 ;肠梗阻组粘膜上皮脱落 ,绒毛坏死 ,粘膜下水肿 ,炎症细胞浸润 ;精氨酸组肠粘膜结构明显改善。结论 :肠梗阻可造成肠粘膜屏障功能损害 ,精氨酸可改善肠粘膜屏障功能 ,减少细菌移位。

Objective:To study the effects of Arginine on the function of intestinal barrier in rats.Methods:Thirty Sprague Dawley rats were divided into three groups randomisely.Arginine 0.25g was given for each rat for five days in Arginine group.Normal saline 1ml/day was injected intra abdominally each rat for five days in intestinal abstruction group and shamoperated group.Then an ileal ligation was performed in Arginine group and intestinal obstruction group.In sham operated group,a laparotomy was given without ligating the ileum.Twenty four hours later,rats were sacrificed and the mesenteric lymph node(MLN),liver,blood and ileum were harvested.Results:1.Bacterial translocation rates in sham operated,intestinal obstruction and Arginine group were 15%,70% and 30% respectively.The rates in intestinal obstruction group were higher than that in sham operated group(P<0.01).The rates in Arginine group were lower than that in intestinal obstruction group (P<0.05).2.Endotoxin levels in sham operated,intestinal obstruction and Arginine group were 0.056±0.003Eu/ml,0.373±0.064Eu/ml and 0.130±0.002Eu/ml respectively.Intestinal obstruction group was higher than that of any other groups and has statistic significance.3.Pathological results:showed that intestinal mucosa in sham operated group was normal.But in intestinal obstruction group,villous necrosis were observed.Epithelial separation and edema can also be seen.In Arginine group,slight epithelial separation and edema were found,but villous necrosis was not observed.Conclusion:1.The mechanical intestinal obstruction may injure the function of intestinal barrier.2.Arginine can decrease the bacterial translocation and the level of endotoxin,and therefore protect intestinal barrier.