银丹心脑通抑制泡沫细胞形成及巨噬细胞分泌基质金属蛋白酶

Yindanxinnaotong Inhibited the Formation of Foam Cells and the Secretion of Matrix Metalloproteinase by Macrophages

目的:银丹心脑临床治疗冠心病心绞痛疗效显著,但其机理尚不明确。本实验通过观察银丹心脑通对巨噬细胞分泌基质金属蛋白酶的作用,探讨银丹心脑通的作用机理。方法:生长状态良好的单核细胞分化成巨噬细胞,加入不同浓度的药物。24 h后收集细胞上清,ELISA检测细胞上清中MMP-2、MMP-9的含量。结果:给药组不同剂量均能抑制MMP-2、MMP-9的表达(P<0.01-0.05),其中高剂量组效果最为显著。结论:银丹心脑通能够抑制巨噬细胞基质金属蛋白酶的分泌,具有明显的抗炎作用,此实验为其在临床上的进一步应用提供了依据。

Objective： Yindanxinnaotong capture has significant efficacy on clinical treatment of angina pectoris,but its mechanism is not clear.The aim of the experiment is to explore action mechanism of yindanxinnaotong capture by observing the effect of macrophage secretion of matrix metalloproteinase.Methods： Good growth monocytes were differentiated into macrophages,and then added drugs with different concentrations.Supernatants were collected after 24 h,Elisa was used to detect the content of MMP-2 and MMP-9.Results： Treatment groups with different doses inhibited MMP-2,MMP-9 expression（P 0.01-0.05）,in which the high-dose group turned out to have the most significant effect.Conclusion： Yindanxinnaotong can inhabit the secretion of matric metalloproteinase and had a significant anti-inflammatory effect.This experiment provided basis for its clinical application.