海星皂甙1对人胶质瘤U87细胞抗增殖作用的研究

Anti-proliferative Effects of Asterosaponin 1 in Human Glioma U87 Cells

目的:海星皂甙是一类从海星中分离、萃取出来的甾体苷类,被认为是海星体内毒素的主要成分。研究表明海星皂甙及其化学衍生物具有多种药理学活性,包括抗菌、抗病毒、抗肿瘤、抑制真菌活性等。本实验旨在研究海星皂甙1对人胶质瘤U87细胞的抗增殖作用和可能的机制。方法:不同浓度海星皂甙1处理人胶质瘤U87细胞后,采用MTT法检测细胞活力,TUNEL染色观察细胞凋亡情况,Westernblot检测内质网应激相关凋亡分子的活性。结果:①海星皂甙1显著抑制U87细胞的增殖,呈时间与剂量依赖性。②海星皂甙1诱导U87细胞发生凋亡。③海星皂甙1处理后U87细胞内质网相关凋亡分子活性明显增高。结论:海星皂甙1通过诱导细胞凋亡抑制人胶质瘤U87细胞的增殖,这种抗增殖作用可能是通过激活内质网应激相关凋亡分子实现的。

Objective： Asterosaponin is a new class of steroidal glycosides extracted and isolated from starfish and responsible for their general toxicity. Diverse pharmacological effects induced by asterosaponin and its analogues have been reported, including antibac- terial, antiviral, antitumor and antifungal activities. The aim of this study is to investigate the potential anti-proliferative activity of aster- osaponin 1 in human glioma U87 ceils, as well as the potential mechanism. Methods： After treatment with different concentrations of asterosaponin 1, the cell viability of human glioma U87 cells was measured by MTT assay. The TUNEL staining was used to detect the apoptotic cells, and westemblot was used to detect the activities of ER related apoptotic factors. Results： （~） Asterosaponin 1 inhibits the proliferation of U87 cells in a dose- and time-depefident manner. （~） Asterosaponin 1 induces apoptotic cell death in human glioma U87 cells. （~） Asterosaponin 1 treated U87 cells exert increased activities ofER-associated apoptotic molecules. Conclusion： Asterosaponin 1 inhibits the proliferation of human glioma U87 cells through induction of apoptotic cell death, which might be mediated by the activating of ER stress-associated apoptotic molecules.