福州荔枝古树胚性愈伤组织限制生长保存研究

Optimization of in vitro Minimal Growth Conservation Conditions of Embryogenic Callus in Ancient Litchi Trees

[目的]探讨荔枝古树胚性愈伤组织的有效保存方法。[方法]以荔枝古树‘宋荔’(编号X2)的胚性愈伤组织为试材,根据蔗糖、甘露醇、肌醇和温度进行正交试验设计,对荔枝古树胚性愈伤组织进行限制生长保存研究。[结果]宋荔X2胚性愈伤组织的最佳保存条件(G7)是MS+2,4-D 1.0 mg/L+蔗糖20 g/L+甘露醇20 g/L+肌醇0.3 g/L,19℃。低温培养有利于提高宋荔X2胚性愈伤组织的细胞活力;宋荔X2胚性愈伤组织生长率与蔗糖浓度呈负相关,但提高蔗糖浓度有利于宋荔X2生长量的增加。不同荔枝古树胚性愈伤组织在G7下均能保存100 d,最长可达135 d。[结论]优化了荔枝古树胚性愈伤组织的保存体系,为进一步完善荔枝古树离体种质资源保存提供了理论基础。

[Objective] The aim was to explore the effective conservation method of EC （embryogenie callus） in ancient litchi trees. [Method] The EC of X2 was as the test materials, orthogonal experiment was designed based on sucrose, mannitol, inositol, and temperature, to study the in vitro restricting conservation in ancient litchi trees. [Result] The best storage condition for X2 EC was on the MS medium with 2,4-D1.0 mg/L ＋ sucrose 20 g/L ＋ mannitol 20 g/L ＋ inositol 0.3 g/L ＋ agar 7 g/L, with the pH value of 5.8, at 19 ℃ in darkness. Low temperature culture conducive to improve the cell viability of embryogenic callus in X2; the growth rate of X2 EC was negatively CmTelated with sucrose concentration, but improving sucrose concentration could increase the growth of X2 EC. Different ancient litchi trees EC in G7 could conserve about 100 d, and the longest conservation life was 135 d. [Conclusion] The results optimized ancient litchi trees EC conservation system, and provided a theoretical basis for improving in vitro conservation of germplasm of ancient litchi trees.