β-环糊精联吡啶钌衍生物与维生素包络作用的荧光光谱分析

Analysis of Binding Properties Between β-Cyclodextrin Bipyridine-ruthenium Derivative and Vitamins by Their Fluorescence Spectra

合成了一种新型的β-环糊精联吡啶钌衍生物[β-CD-Ru(bpy)3],采用分子荧光光谱法研究了该衍生物对一系列维生素的包络作用.β-CD-Ru(bpy)3的结构中含有发光活性基团联吡啶钌中心,因此具有良好的荧光特性.当加入与β-环糊精有主客体识别作用的维生素时,β-CD-Ru(bpy)3与维生素之间发生能量转移从而导致主体分子发生荧光猝灭.采用Benesi-Hildebrand方程,根据其猝灭值计算了该衍生物与各种维生素的包络常数.实验结果表明,维生素与β-CD-Ru(bpy)3包络作用大小顺序为维生素B12>维生素K3>维生素C>维生素A.

This paper presents a research of the binding interaction of a new synthetized β-cyclodextrin bipyridine-ruthenium derivative[β-CD-Ru（bpy）3] with a series of vitamins using fluorescent spectrometry. The performance of fluorescence emission of β-CD-Ru（bpy）3 was excellent due to the existence of the center of bipyridine-ruthenium. Based on the effect of molecular recognition between β-cyclodextrin and guest molecule, the fluorescence quenching occured because of the energy transfer between the β-CD-Ru（bpy）3 and the vitamin when a kind of vitamin was added. According to the quantitative value of fluorescence quenching, the binding constants of the new derivative with respect to various vitamins were determined by Benesi-Hildebrand Equation. The results show that the binding capacities of the vitamins with β-CD-Ru（bpy）3 were in a descending sequence of VB12〉VK3〉VC〉VA.