子宫内膜癌和子宫平滑肌瘤内皮抑素及CD34内皮祖细胞的表达及其意义

Study on the expression of endostatin and CD34 endothelial progenitor cells (EPCs) and its significance in endometrial adenocarcinoma and uterus myoma

目的研究子宫内膜癌和子宫平滑肌瘤组织内皮抑素、内皮祖细胞(EPCs)的表达及其临床意义。方法应用免疫组化方法检测24例正常子宫内膜、68例子宫肌瘤和92例子宫内膜癌组织中内皮抑素及EPCs的表达,评价其与子宫内膜癌临床病理分期及病情发展的相关性。结果①内皮抑素蛋白的表达在正常子宫内膜与子宫平滑肌瘤之间无显著性差异,子宫内膜癌组织中内皮抑素表达明显高于正常子宫内膜组和子宫平滑肌瘤组。②子宫内膜癌CD34 EPCs表达与正常子宫内膜及子宫平滑肌瘤比较均有显著性差异。③临床期别越高,内皮抑素的表达越高,内皮抑素表达与临床手术分期相关(P<0.05)。④临床期别越高,组织分化越差,EPCs表达越高。临床手术分期、组织学分级、淋巴结转移和肌层浸润均与CD34 EPCs表达有关。⑤内皮抑素与EPCs的表达有显著相关性(r=0.708,P<0.01),内皮抑素水平越高,EPCs表达越多。结论子宫内膜癌组织内皮抑素和CD34 EPCs表达水平与子宫内膜癌的发生发展及患者预后有关。

Objective It is to investigate the expression of endostatin and endothelial progenitor cells（EPCs） and its significance in endometrial adenocarcinoma and uterus myoma.Methods The expression of endostatin and EPCs in 24 cases of normal endometrium,68 cases of uterus myoma and 92 cases of endometrial adenocarcinoma were examined by immunohistochemisry staining,and the correlations of them with the clinical pathological parameters and progression of disease with adenocarcinoma endometrial were analyzed.Results ①There was no significant difference in the expression of endostatin protein between normal endometrium and uterus myoma,but the expression of endostatin protein in endometrial adenocarcinoma was markedly higher than that in uterus myoma and normal endometrium.②There was significant difference in the expression of EPCs between endometrial adenocarcinoma and normal endometrium or uterus myoma.③The levels of endostatin protein expression in adenocarcinoma endometrial was significantly associated with clinical stage.④The levels of EPCs in adenocarcinoma endometrial was significantly associated with clinical stage,histological grades,lymph node metastasis and myometrial invasion.⑤The expression of endostatin was significantly associated with EPCs.Conclusion The expression of endostatin protein and EPCs may has a relationship with the development of endometrial adenocarcinoma and prognosis of the patients.