应用Volume软件测定肺减容动物实验中肺容积变化的研究

Volume software measuring pulmonary volume of lung volume reduction in animal experiment

目的探讨多层螺旋CT定量分析Volume软件在肺减容术动物实验中肺容积测定的应用价值。方法 8头健康约克幼猪,随机分为实验组、对照组各4头,经纤维支气管镜行生物性肺减容术,采用多层螺旋CT扫描,Volume软件动态监测术前、术后1周,术后1,3个月肺容积变化,分析其与术后3个月取出的动物肺脏其形态学,组织病理学的关联性结果对照组4头幼猪,右肺容积/总肺容积平均比值由术前(48.9±4.7)%到术后3个月减至(48.2±5.7)%,P>0.05,差异无显著性。实验组4头幼猪各右肺容积/总肺容积比值术后较术前均明显减小,其平均比值由术前(57.9±1.6)%到术后3个月减至(49.6±2.65)%,P<0.05,差异有显著性。同时,对照组中术前、术后的影像学、3个月后取出靶区肺的大体形态及病理学上均无明显改变,而实验组4头幼猪3个月后肺靶区体积明显减少,均有不同程度的肺纤维化改变,与Volume软件检测的肺容积变化相一致。结论多螺旋CTVolume软件为肺减容术动物实验中肺容积测定提供了一种简单,无创,迅速,准确地估测方法 。

[Objective] To evaluate the value of volume software of multi-slice spiral CT in measuring pulmonary volume of lung volume reduction in animal experiment.[Methods] Eight young pigs （16～22 kg） were randomly divided into experimental group and control group.Each pig was treated with biological lung volume reduction under fiberoptic bronchoscope guide before operation and one week after,one month and three month after the operation,its lung volume was measured respectively by volume software,then relation with its morphology and histopathology was analyzed and the effectiveness of lung volume reduction was evaluated.[Result] The control group with 4 young pigs whose right lung volume /total lung declined from （48.9±4.7）% to （48.2±5.7）% after three months were no significant differences （P 0.05）.But there were straightly reduced from （57.9±1.6）% to （49.6±2.65）% in experimental group with other 4 young pigs （P 0.05）.Meanwhile three months later its volume of target lung dramatically decreased,following pulmonary fibrosis in varying degrees,which extremely consisted with the imaging changes.Yet the control group with 4 pigs had no significant changes in morphology and histopathology,even preoperative and postoperative CT scans.[Conclusion] It is a simple,rapid,accurate and no trauma method estimated by volume software supplied by multi-slice spiral CT for measuring pulmonary volume of lung volume reduction in animal experiments.