摘要
现代分子生物学技术和新的统计方法,大大促进了植物数量性状基因(QTL)的解析。连锁作图和关联作图是目前植物数量性状基因解析的重要方法,两者在QTL定位的精度和广度、提供的信息量、统计分析方法等方面具有明显的互补性。连锁作图可以初步对目标性状基因进行定位,而关联作图则可快速对目标基因进行验证和精细定位,并针对特定候选基因提供大量信息,验证候选基因的功能。综述了近10年来棉花数量性状的连锁作图和关联作图最新进展,并结合两种方法对棉花的QTL研究前景进行了展望。
Both modem molecular biology technique and new statistical methods greatly promoted the analysis of plant quantitative trait loci (QTL). Linkage mapping (LM)and association mapping (AM) are two important methods for QTL analysis. Both them have obvious complementarity in the accuracy and breadth of QTL mapping, the provided information, and the statistical analysis method. Linkage mapping earl preliminary locate the target trait gene, while association mapping can quickly achieve the verification and fine mapping of target gene; moreover, it can also verify the candidate gene function according to a lot of information provided for a specific candidate gene. In this paper, the new advances were reviewed from the linkage mapping and association mapping of cotton quantitative traits. The research prospects of cotton QTL were analyzed by combining the two methods.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第11期130-133,139,共5页
Guangdong Agricultural Sciences
基金
国家"863"计划项目(2012AA101108)
国家转基因生物新品种培育重大专项(2012ZX08013007-001-004)
河南省重点科技攻关计划(092102110025
112102110105)
关键词
棉花
QTL
连锁作图
关联作图
cotton
QTL
linkage mapping
association mapping