曼氏迭宫绦虫线粒体载体蛋白结构和功能的生物信息学分析

Bioinformatics analysis for structure and function of mitochondrial carrier protein superfamily from Spirometra mansoni

目的应用生物信息学技术预测曼氏迭宫绦虫线粒体载体蛋白超家族的结构和功能,为进一步研究曼氏迭宫绦虫的蛋白质提供理论依据。方法将测得的曼氏迭宫绦虫成虫EST序列用ORF finder获取开放读码框,Blast进行分析其结构域。应用分析工具Protparam、InterProScan、protscale、SignalP-3.0、PSORTⅡ、BepiPred、TMHMM、VectorNTI Suite 9软件包、Phyre2分别进行该蛋白质的基本性质、结构域、疏水性、信号肽、亚细胞定位、抗原表位、跨膜区及空间结构的预测及分析。结果 Blast预测该蛋白质为线粒体载体蛋白超家族,保守功能域为线粒体二羧酸载体,含294个氨基酸残基,理论分子量为32132.2Da。与曼氏血吸虫进化地位最接近;有1个信号肽位点和6个潜在的抗原表位。结论曼氏迭宫绦虫线粒体二羧酸载体能够介导苹果酸、琥珀酸等二羧酸的转运,使其跨越线粒体膜参与三羧酸循环,为虫体自身提供能量,可能是潜在的疫苗候选分子及药物作用靶点。

Objective To study the structure and function ofmitochonddal carder protein superfamily from Spirometra mansoni by bioinformatics technology, and to provide a theoretical basis for further study. Methods Open reading frame （ORF） of EST sequence from Spirometra manoni was obtained by ORF finder and was translated into amino acid by DNAclub. The structure domain was analyzed by Blast. By the method of online analysis tools： Prot- param, InterProScan, protscale, SignalP-3.0, PSORT II, BelSiPred, TMHMM, VectorNTI Suite 9 packages and Phyre2, the structure and function of the protein were predicted and analyzed. Results The mitochondrial carder protein su- perfamily may be Spirometra mansoni mitoehondrial dicarboxylate carrier （Sin DIC）. The sequence contained 294 ami- no acid residues and its theoretical molecular weight was 32003.4 Da. It had three full conserved functiOnal domains that was mito-carr superfamily, with the closest to evolutionary position of Schistosoma manoni. It had a signal pep- tide site and six potential antigen epitopes. Conclusion Sm DIC can mediate malic acid, succinic acid and other 2-carboxylic acid to across the mitochondrial membrane to participate in the citric acid cycle, providing energy for the parasites. Sm DIC may be a potential vaccine candidate molecules and a possible target for drug action.