腺病毒介导表皮生长因子体外转染人牙髓干细胞对其凋亡的影响

Study on the apoptosis of human dental pulp stem cells transfected by recombinant adenovirus mediated epidermal growth factor in vitro

目的通过体外培养人牙髓干细胞(Human dental pulp stem cells,hDPSCs),观察重组腺病毒介导的表皮生长因子(Recombinant adenovirus mediated epidermal growth factor,rAd-EGF)转染至hDPSCs后对其凋亡的影响。方法 rAd-EGF转染hDPSCs后,采用RT-PCR检测EGF mRNA的表达情况,用流式细胞仪Annexin V-FITC/PI法检测hDPSCs的凋亡情况。结果在一定时间内,rAd-EGF转染入hDPSCs,实验组hDPSCs中EGF mRNA的表达水平相对于阴性对照组与空白组均明显上调(P<0.05),并且同阴性对照组与空白组比较,实验组hDPSCs体外凋亡指数明显降低(P<0.05)。结论 rAd-EGF转染入hDPSCs,EGF mRNA的表达量增加,在短期内对其凋亡具有抑制作用。

Objective To observe the effect of recombinant adenovirus mediated epidermal growth factor （rAd-EGF） transfecting the human dental pulp stem cells （hDPSCs） on its apoptosis by culturing human dental pulp stem cells in vitro. Methods After used rAd-EGF to transfect hDPSCs, reverse transcription polymerase reaction （RT-PCR） was used to analyze the mR.NA expression of EGF in hDPSCs, and flow cytometry Annexin V-FITC/PI was used to detect the effect of the apoptosis of hDPSCs. Results Within a certain time, rAd-EGF was transfected into hDPSCs. The EGF mRNA expression of hDPSCs in the experimental group were significantly up-regnlated compared with the negative control group and blank control group （P〈0.05）. And compared with the negative control group and the blank group, the apoptotic index of hDPSCs in the experimental group was significantly lower （P〈0.05）. Conclusion The rAd-EGF could be transfected into hDPSCs successfully, and the EGF mRNA expression of hDP- SCs was increased. In the short term, the rAd-EGF transfected into hDPSCs could inhibit apoptosis.