宫颈癌组织中ZEB1表达及其临床意义

The expression of ZEB1 in carcinoma of the cervix and its significance

目的探讨ZEB1在宫颈癌组织中的表达及其临床意义。方法采用免疫组织化学SP法和蛋白质印迹分析方法对郑州人民医院妇科2008年9月至2012年4月收治的宫颈癌59例、宫颈上皮内瘤变组织(CIN)43例和正常宫颈组织30例中ZEB1表达情况进行分析,并观察其与宫颈癌组织临床病理特征的关系。结果 ZEB1在宫颈癌组织中的表达高于CINⅡ～Ⅲ组织,而CINⅠ组织表达高于正常宫颈组织(P<0.05)。宫颈癌组织阳性表达率[93.22%(55/59)]高于CINⅡ～Ⅲ组织中阳性表达率[65.52%(19/29)]、CINⅠ组织阳性表达率[42.86%(6/14)]及正常宫颈组织(ZEB1不表达)。蛋白质印迹分析显示ZEB-1蛋白在宫颈癌组织中的表达高于CINⅡ～Ⅲ组织、CINⅠ组织和正常宫颈组织(P<0.05)。ZEB1的表达强度与临床分期、淋巴转移、浸润宫颈深度及肿瘤分化程度等因素相关(P<0.05)。结论 ZEB1在宫颈癌中高表达,提示其与宫颈癌的发生、发展相关,有可能参与宫颈癌细胞上皮间质转化而促进宫颈癌的侵袭和转移。

Objective To investigate the expression of ZEB-I in cervical cancer and its significance. Methods The im- munohistoehemical method and western blotting was used to detect the expression of ZEB-1 in 59 cervical cancer, 43 cervical intra-epithelial neoplasia （CIN）and 30 normal cervical tissues. The relationship between ZEB-1 protein expression and clinicopathologieal features of cervical cancer was evaluated. Results Immunohistoehemical staining showed that the positive rates of ZEB-1 protein expression in cervical cancer tissues 93.22% （55/59）, CIN Ⅱ～Ⅲ65. 52% （19/29）, CIN Ⅰ 42. 86% （6/14） and normal （ 0 ）. respectively. Western blotting analysis showed that ZEB-1 protein expression in cervical cancer tissues was significantly higher than those in the paracaneerous cervix tissues and normal cervix tissues （ P 〈 0.05 ） . We also found that ZEB-1 expression was significantly correlated with the clinical stage, Lymphatic metastasis, infiltrate cervix depth and differentiation degree postoperative recurrence （ P 〈 0.05 ）. Conclusion High expression of ZEB -1 is correlated with the development and progression of cervical cancer. ZEB-1 might cancer participate in the epithelial -mesenehymal transition of cervical cancer cells, subsequently contributing to the invasion and metastasis.