HOXA10基因在不明原因不孕患者子宫内膜组织中的表达及其临床意义

Expression and clinical significance of HOXA10 gene in the unexplained infertility endometrium

目的探讨HOXA10基因在正常妇女及不明原因不孕患者子宫内膜中的表达及临床意义。方法选取2010年12月至2011年10月期间在新疆医科大学第一附属医院生殖助孕中心就诊的38例对照组妇女及55例不明原因不孕患者,留取分泌期子宫内膜组织。分别采用原位杂交和实时荧光定量PCR技术检测各组子宫内膜组织中HOXA10mRNA表达水平。结果原位杂交法检测显示,对照组中分泌中晚期子宫内膜间质细胞中HOXA10mRNA表达量高于腺上皮细胞(P<0.05);HOXA10mRNA在对照组分泌期上皮细胞、间质细胞中表达量均明显高于不明原因不孕组(P<0.05)。实时荧光定量PCR技术检测显示,HOXA10mRNA在对照组分泌中晚期表达显著高于分泌早期(P<0.01);不明原因不孕患者HOXA10mRNA分泌中晚期高于分泌早期(P<0.05)。不明原因不孕患者子宫内膜HOXA10mRNA基因表达均显著低于对照组(P<0.01)。结论 HOXA10基因在不明原因不孕子宫内膜组织中低表达,可能是影响子宫内膜容受性导致着床障碍的不孕原因之一。

Objective To investigate the HOXA10 gene expression in the endometrium of unexplained infertility women. Methods Between Dec. 2010 and Oct. 2011,55 patients with unexplained infertility and 38 patients as control groupwere selected to get endometrium in the secretory phase. Results By in situ hybridization, Patients with controls havehigher HOXAlOmRNA levels in the epithelial cells and stromal cells of endometrium as compared to unexplained infertil-ity during the menstrual cyele （ P 〈0. 05） . By real-time fluorescent quantitation technique,Patients with controls havehigher HOXA10 mRNA levers in mid-and late-sceretary endometrium as compared to early sceretary phase （ P 〈0. 01 ）;Patients with unexplained infertility have higher HOXAlOmRNA levers in mid-and late-sceretary endometrium as com-pared to early sceretary phase（ P 〈0. 05） ; Patients with unexplained infertility have lower HOXAlOmRNA levers in mid-and late-sceretary endometrium as compared to controls （ P 〈0.01）. Conclusion HOXA10 gene in patients with unex-plained infertility uterine endometrial tissues were low expression, may be associated with implantation disorders,leadingcause of infertility.