解脲脲原体和人型支原体体外12种抗生素耐药性分析

Analysis of Ureaplasma urealyticum and Mycoplasma hominis resistance to 12 types of antibiotics in vitro

目的分析解脲脲原体(Uu)和人型支原体(Mh)所引起的泌尿生殖道感染对12种抗生素的耐药性,掌握其规律,以便正确引导临床合理使用抗生素。方法正确采集泌尿生殖道感染患者标本,男性:用男性拭子由前尿道1～2cm处取尿道分泌物;女性:取宫颈管内1～2cm处宫颈分泌物。采用珠海迪尔生物工程有限公司生产的支原体分离培养药敏试剂盒进行检测;同时用法国生物梅里埃A7支原体分离培养基进行确认;以上培养结果相符者,再做药敏分析。结果单一Uu感染者对环丙沙星耐药率高达70.70%,对交沙霉素和克拉霉素敏感率最高,分别为100.00%、'%。单一Mh感染者,对阿奇霉素、红霉素和罗红霉素耐药率最高,均为93.75%;而对强力霉素、美满霉素和交沙霉素的敏感率最高,均为100.00%。Uu和Mh混合感染者,对红霉素、罗红霉素、阿奇霉素、克拉霉素和环丙沙星的耐药率较高,分别为96.53%、95.83%、94.44%、90.97%和88.89%,交沙霉素的敏感率最高为98.61%。结论不同的支原体感染类型,在抗生素敏感性上不尽相同,因而临床应根据药敏结果规范用药,以达到最佳的治疗效果。

Objective To analyze Ureaplasma urealyticum （Uu） and Mycoplasma hominis （Mh） caused by in- fection of urogenital tract of 12 antibiotics,master the rules,so as to provide proper guidance for clinical appliance of antibiotics. Methods Correctly collected specimens from patients with urogenital tract infection. Male patients：col- lected urethral secretions at 1-2 cm of anterior urethra with male swab; female patients：collected cervical secretions at 1-2 cm inside of cervical canals. Test with mycoplasma isolated culture sensitivity test kit producted by Zhuhai DL Medical Biotech Co. , Ltd, and identified with A7 mycoplasma isolation culture medium made by Bio Merieux. Test drug sensitivity of mycoplasma cultured and isolated on above. Results A single Uu infection on ciprofloxacin re- sistance rate reached up to 70. 70%,josamycin and clarithromycin sensitive rate was highest, respectively 100%, 98.80%. Of patients infected by Mh only,resistance rates to azithromycin, erythromycin and roxithromycin were the highest,all reach 93. 75%, the sensitivity to deoxycycline, minocycline and josamycin were the highest, all reach 100.00%. Of patients infected by both Uu and Mh, resistance to erythromycin, roxithromycin, azithromycin, clarithromycin and ciprofloxacin were the highest at 96. 53%, 95. 83%, 94.44%,90. 97% and 88. 89%, sensitivity to josamycin was the highest at 98.61%. Conclusion Different types of mycoplasma infection is not the same as in the antibiotic sensitivity, consequently, clinical appliance of antibiotics should be based on result of drug sensitivity test, so as to gain the best result in clinical therapy.